Study of physically adsorbed stationary phases for open tubular capillary electrochromatography

	Study of physically adsorbed stationary phases for open tubular capillary electrochromatography
	Liu, Z; Zou, HF; Ye, ML; Ni, JY; Zhang, YK
刊名	electrophoresis
	1999-10-01
卷号	20 期号:14 页码:2891-2897
关键词	open tubular capillary electrochromatography adsorption protein peptide amino
英文摘要	a novel method based on the adsorption of positively charged compounds on the wall of a fused-silica capillary was applied to prepare stationary phases for open tubular capillary electrochromatography (otcec). the positively charged substances including cationic surfactant such as cetyltrimethylammonium bromide (ctab) and basic chiral selectors such as protein, peptide and amino acid were physically adsorbed onto the capillary wall under specially selected conditions. the adsorbed stationary phase of ctab was used to separate neutral compounds, while the others were used for chiral separations. the run-to-run reproducibility of retention time was rather good with relative standard deviation (rsd) values of less than 2.3%. the separation efficiency was excellent with the highest theoretical plate number of up to 590 000/m and the average one above 250 000/m. stored at 2-8 degrees c in the refrigerator, the adsorbed stationary phase can last at least one month. it was observed that the uv spectra for the enantiomers are significantly different due to the diastereomeric interactions of enantiomers with the chiral stationary phase in the detection window. with the use of the same capillary, the same instrument, and the same mobile phase, the superiority of otcec over open tubular liquid chromatography (otlc) and capillary zone electrophoresis (cze) was illustrated.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	fused-silica capillaries ; flight mass detector ; liquid-chromatography ; electrophoretic techniques ; enantiomer separation ; gradient elution ; selectivity ; resolution ; flow ; performance
收录类别	SCI
语种	英语
WOS记录号	WOS:000085044200016
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/138542]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Lab Chromatog, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Liu, Z,Zou, HF,Ye, ML,et al. Study of physically adsorbed stationary phases for open tubular capillary electrochromatography[J]. electrophoresis,1999,20(14):2891-2897.
APA	Liu, Z,Zou, HF,Ye, ML,Ni, JY,&Zhang, YK.(1999).Study of physically adsorbed stationary phases for open tubular capillary electrochromatography.electrophoresis,20(14),2891-2897.
MLA	Liu, Z,et al."Study of physically adsorbed stationary phases for open tubular capillary electrochromatography".electrophoresis 20.14(1999):2891-2897.