Rapid separation of nucleosides by capillary electrochromatography with a methacrylate-based monolithic stationary phase

	Rapid separation of nucleosides by capillary electrochromatography with a methacrylate-based monolithic stationary phase
	Ping, GC ; Zhang, WB ; Zhang, LH ; Schmitt-Kopplin, P ; Zhang, YK ; Kettrup, A
刊名	chromatographia
	2003-05-01
卷号	57 期号:9-10 页码:629-633
关键词	capillary electrochromatography monolithic column nucleosides
英文摘要	separation of nucleosides by capillary electrochromatography with a methacrylate-based monolithic column is described. because the nucleosides are relatively hydrophilic compounds, chromatographic partition is very weak on non-polar stationary phases. a small quantity of organic modifier is usually required to increase their retention factors but, because the wettability of the stationary phase by the mobile phase is rather poor, bubble formation can be a significant problem. this situation was improved by use of a polymeric monolithic column with higher level charged monomer in the polymerization mixture, because of the presence of charged moieties on the surface of the monolithic stationary phase. the very high stability under conditions of very low organic modifier concentration used in the experiment showed that the wettability of the stationary phase by the mobile phase was satisfactory. the effect of ph, organic modifier content, and applied voltage on the separation was investigated. baseline separation of six nucleosides was achieved within 4.5 min on this type of column. because of the presence of charged moieties and butyl groups on the surface of the monolithic column, the mechanism of separation might involve both ion-exchange and hydrophobic interactions.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	performance liquid-chromatography ; rigid polymer monoliths ; columns ; media
收录类别	SCI
语种	英语
WOS记录号	WOS:000183320400010
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/138390]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China 2.GSF Natl Ctr Environm & Hlth, Inst Ecol Chem, D-85764 Neuherberg, Germany
推荐引用方式 GB/T 7714	Ping, GC,Zhang, WB,Zhang, LH,et al. Rapid separation of nucleosides by capillary electrochromatography with a methacrylate-based monolithic stationary phase[J]. chromatographia,2003,57(9-10):629-633.
APA	Ping, GC,Zhang, WB,Zhang, LH,Schmitt-Kopplin, P,Zhang, YK,&Kettrup, A.(2003).Rapid separation of nucleosides by capillary electrochromatography with a methacrylate-based monolithic stationary phase.chromatographia,57(9-10),629-633.
MLA	Ping, GC,et al."Rapid separation of nucleosides by capillary electrochromatography with a methacrylate-based monolithic stationary phase".chromatographia 57.9-10(2003):629-633.