Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxy-2 '-deoxyguanosine

	Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxy-2 '-deoxyguanosine
	Mei, SR ; Yao, CH ; Cai, LS ; Xing, J ; Xu, GW ; Wu, CY
刊名	electrophoresis
	2003-05-01
卷号	24 期号:9 页码:1411-1415
关键词	cancer capillary electrophoresis end-column amperometric detection 8-hydroxy-2 '-deoxyguanosine urine
英文摘要	urinary 8-hydroxy-2'-deoxyguanosine (80hdg) is an excellent marker of oxidative dna damage. until now, urinary 80hdg has been measured by high-performance liquid chromatography with electrochemical detection. a simple and sensitive method for the analysis of urinary 80hdg by capillary electrophoresis with end-column amperometric detection has been developed in our laboratory. a single-step solid-phase extraction procedure was optimized and used for extracting 80hdg from human urine. to improve the sensitivity of this method, a new focusing technique based on a dynamic ph junction was used. the limit of detection was 20 nm (signal-to-noise ratio s/n = 3), the linear range was 50 nm-10 mum, and the correlation coefficient was better than 0.999. the relative standard deviation (rsd) was found to be 0.57% for migration time, and 4.79% for peak current. to show the usefulness of the method, the urinary concentration of 80hdg in nine healthy persons and ten cancer patients was determined. the urinary concentration of 80hdg in cancer patients was significantly higher than that in healthy persons.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	oxidative dna-damage ; dynamic ph junction ; electrochemical detection ; gas-chromatography ; 8-hydroxydeoxyguanosine ; mutations ; biomarker ; assay ; base
收录类别	SCI
语种	英语
WOS记录号	WOS:000182965000011
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/138353]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, R&A Ctr, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China 2.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Mei, SR,Yao, CH,Cai, LS,et al. Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxy-2 '-deoxyguanosine[J]. electrophoresis,2003,24(9):1411-1415.
APA	Mei, SR,Yao, CH,Cai, LS,Xing, J,Xu, GW,&Wu, CY.(2003).Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxy-2 '-deoxyguanosine.electrophoresis,24(9),1411-1415.
MLA	Mei, SR,et al."Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxy-2 '-deoxyguanosine".electrophoresis 24.9(2003):1411-1415.