Determination of spinosin in rat plasma by reversed-phase high-performance chromatography after oral administration of Suanzaoren decoction

	Determination of spinosin in rat plasma by reversed-phase high-performance chromatography after oral administration of Suanzaoren decoction
	Li, YJ ; Liang, XM ; Xiao, HB ; Bi, KS
刊名	journal of chromatography b-analytical technologies in the biomedical and life sciences
	2003-04-25
卷号	787 期号:2 页码:421-425
关键词	spinosin
英文摘要	a sensitive, simple, and accurate method for determination of spinosin in rat plasma with sulfamethoxazole (smz) as internal standard was developed using rp-hplc with uv detection. sample preparations were carried out by protein precipitation with acetonitrile, followed by the evaporation of the acetonitrile to dryness. the resultant residue was then reconstituted in mobile phase and injected onto a hypersil c-18 (200x4.6 mm i.d., 5 mum) analytical column. the mobile phase consisted of acetonitrile-water (15:85, v/v) with 1% glacial acetic acid. the assay was shown to be linear over the range of 18.07-903.5 ng/ml (r-2=0.995). mean recovery was determined as 93.6%. within- and between-day precisions were less than or equal to8.9% rsd. the limit of quantitation was 18.07 ng/ml. the hplc method developed has been applied to determine the pharmacokinetics of spinosin in rat plasma after having taken suanzaoren decoction. (c) 2002 elsevier science b.v. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
收录类别	SCI
语种	英语
WOS记录号	WOS:000182055300018
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/138348]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China
推荐引用方式 GB/T 7714	Li, YJ,Liang, XM,Xiao, HB,et al. Determination of spinosin in rat plasma by reversed-phase high-performance chromatography after oral administration of Suanzaoren decoction[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2003,787(2):421-425.
APA	Li, YJ,Liang, XM,Xiao, HB,&Bi, KS.(2003).Determination of spinosin in rat plasma by reversed-phase high-performance chromatography after oral administration of Suanzaoren decoction.journal of chromatography b-analytical technologies in the biomedical and life sciences,787(2),421-425.
MLA	Li, YJ,et al."Determination of spinosin in rat plasma by reversed-phase high-performance chromatography after oral administration of Suanzaoren decoction".journal of chromatography b-analytical technologies in the biomedical and life sciences 787.2(2003):421-425.