Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxydeoxyguanosine of cancer patients

	Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxydeoxyguanosine of cancer patients
	Mei, SR ; Cai, LS ; Yao, QH ; Xing, J ; Xu, GW ; Wu, CY
刊名	chemical journal of chinese universities-chinese
	2003-11-01
卷号	24 期号:11 页码:1987-1989
关键词	8-hydroxydroxydeoxyguanosine capillary electrophoresis with end-column amprometric detection method dynamic pH junction injection cancer
英文摘要	using one step solid-phase extraction for urine pretreatment and a new sample focusing mode-dynamic ph junction injection for increasing sensitivity, a simple and sensitive method for the analysis of urinary 8ohdg by capillary electrophoresis with end-column amprometric detection has been developed. the limit of detection was 20 nmol/l (signal to noise ratio s/n=3). the urinary concentration of 8ohdg in nine healthy persons and 28 cancer patients was determined. it was found that urinary concentration of 8ohdg in cancer patients was significantly higher than that in healthy persons[(35. 26+/-27. 96) nmol/l) vs. (13. 51+/-5. 08) nmol/l, p<0. 05], the result demonstrated that 8ohdg may be a good biomarker for measurement of oxidative dna damage in cancer patients. furthermore, the excretion levels of urinary 8ohdg from cancer patients receiving surgical therapy are investigated, and the results demonstrated that the smoking has a strong effect on 8ohdg content.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, multidisciplinary
研究领域[WOS]	chemistry
关键词[WOS]	oxidative dna-damage ; performance liquid-chromatography ; electrochemical detection ; base
收录类别	SCI
语种	英语
WOS记录号	WOS:000186799000016
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/138321]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China 2.Acad Sinica, Dalian Inst Chem Phys, Analyt Ctr State Chromatog Res, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Mei, SR,Cai, LS,Yao, QH,et al. Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxydeoxyguanosine of cancer patients[J]. chemical journal of chinese universities-chinese,2003,24(11):1987-1989.
APA	Mei, SR,Cai, LS,Yao, QH,Xing, J,Xu, GW,&Wu, CY.(2003).Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxydeoxyguanosine of cancer patients.chemical journal of chinese universities-chinese,24(11),1987-1989.
MLA	Mei, SR,et al."Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxydeoxyguanosine of cancer patients".chemical journal of chinese universities-chinese 24.11(2003):1987-1989.