Optimized preparation of poly(styrene-co-divinylbenzene-co-methacrylic acid) monolithic capillary column for capillary electrochromatography

	Optimized preparation of poly(styrene-co-divinylbenzene-co-methacrylic acid) monolithic capillary column for capillary electrochromatography
	Jin, WH; Fu, HJ; Huang, XD; Xiao, H; Zou, HF
刊名	electrophoresis
	2003-09-01
卷号	24 期号:18 页码:3172-3180
关键词	basic pharmaceuticals capillary electrochromatography hormones peptides stationary phase styrene-based monolith
英文摘要	preparation of a poly(styrene-co-divinylbenzene-co-methacrylic acid) monolithic stationary phase for the use in capillary electrochromatography (cec) has been improved by optimizing the polymerization conditions. it is observed that the reaction time strongly affects column efficiency, while the proportion of isooctane in porogen influences peak symmetry of some solutes seriously. the lifetime of the monolithic columns prepared mainly depends on the ph of buffers used. reproducibility of electroosmotic flow (eof) from batch to batch columns are lower than 2.8% relative standard deviation. unlike other types of capillary electrochromatographic monoliths, a ph-dependent eof was observed on this type of column. separation of various types of compounds including aromatic hydrocarbons, hormones, anilines, basic pharmaceuticals, and peptides was achieved. the facile preparation and wide application of this monolithic column may make styrene-based polymer a potential stationary phase in cec.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	rigid polymer monoliths ; reversed-phase electrochromatography ; stationary phases ; liquid-chromatography ; separation media ; packed capillaries ; porous properties ; surface-chemistry ; peptides ; flow
收录类别	ISTP ; SCI
语种	英语
WOS记录号	WOS:000185897100011
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/138320]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Jin, WH,Fu, HJ,Huang, XD,et al. Optimized preparation of poly(styrene-co-divinylbenzene-co-methacrylic acid) monolithic capillary column for capillary electrochromatography[J]. electrophoresis,2003,24(18):3172-3180.
APA	Jin, WH,Fu, HJ,Huang, XD,Xiao, H,&Zou, HF.(2003).Optimized preparation of poly(styrene-co-divinylbenzene-co-methacrylic acid) monolithic capillary column for capillary electrochromatography.electrophoresis,24(18),3172-3180.
MLA	Jin, WH,et al."Optimized preparation of poly(styrene-co-divinylbenzene-co-methacrylic acid) monolithic capillary column for capillary electrochromatography".electrophoresis 24.18(2003):3172-3180.