Membrane supports as the stationary phase in high performance immunoaffinity chromatography

	Membrane supports as the stationary phase in high performance immunoaffinity chromatography
	Zhou, DM; Zou, HF; Ni, JY; Yang, L; Jia, LY; Zhang, Q; Zhang, YK
刊名	analytical chemistry
	1999
卷号	71 期号:1 页码:115-118
英文摘要	the membrane with a composite of cellulose grafted with acrylic polymers formed by polymerizing a glycidyl methacrylate in the presence of dispersed cellulose fiber was prepared as the stationary phase; the column (40 x 4 mm i.d.) which was compatible with the hplc instrument was packed with pieces of the cut membrane. protein a and human igg were immobilized on the membrane stationary phase. the column based on the membrane support provided us good reproducibility, high efficiency, and low back pressure. high-performance immunoaffinity chromatographic analysis of human igg in serum and polyclonal antibody to human igg raised in goat was performed within 2.5 min. the fast-speed immunoaffinity analysis was developed by increasing the now rate of the mobile phase and decreasing the duration time for the switch of the mobile phase; an operation for immunoaffinity analysis of human igg could be finished within 30 s.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	perfusion chromatography ; affinity-chromatography ; ion-exchange ; purification ; proteins ; separation ; antibody
收录类别	SCI
语种	英语
WOS记录号	WOS:000077925500026
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/138205]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Zhou, DM,Zou, HF,Ni, JY,et al. Membrane supports as the stationary phase in high performance immunoaffinity chromatography[J]. analytical chemistry,1999,71(1):115-118.
APA	Zhou, DM.,Zou, HF.,Ni, JY.,Yang, L.,Jia, LY.,...&Zhang, YK.(1999).Membrane supports as the stationary phase in high performance immunoaffinity chromatography.analytical chemistry,71(1),115-118.
MLA	Zhou, DM,et al."Membrane supports as the stationary phase in high performance immunoaffinity chromatography".analytical chemistry 71.1(1999):115-118.