Metabolic profiling based on LC/MS to evaluate unintended effects of transgenic rice with cry1Ac and sck genes

	Metabolic profiling based on LC/MS to evaluate unintended effects of transgenic rice with cry1Ac and sck genes
	Chang, Yuwei 1; Zhao, Chunxia 1; Zhu, Zhen 2; Wu, Zeming 1; Zhou, Jia 1; Zhao, Yanni 1; Lu, Xin 1; Xu, Guowang 1
刊名	plant molecular biology
	2012-03-01
卷号	78 期号:4-5 页码:477-487
关键词	Liquid chromatography Mass spectrometry Metabolic profiling Transgenic rice Unintended effects Metabolomics
英文摘要	as a primary characteristic of substantial equivalence, the evaluation of unintended effects of genetically modified plants has been evolving into an important field of research. in this study, a metabolic profiling method for rice seeds was developed using rapid resolution liquid chromatography/quadrupole time-of-flight mass spectrometry. the analytical properties of the method, including the linearity, reproducibility, intra-day precision and inter-day precision, were investigated and were found to be satisfactory. the method was then applied to investigate the differences between transgenic rice and its native counterparts, in addition to the differences found between native rice with different sowing dates or locations. global metabolic phenotype differences were visualized, and metabolites from different discriminated groups were discovered using multivariate data analysis. the results indicated that environmental factors played a greater role than gene modification for most metabolites, including tryptophan, 9,10,13-trihydroxyoctadec-11-enoic acid, and lysophosphatidylethanolamine 16:0. the concentrations of phytosphingosine, palmitic acid, 5-hydroxy-2-octadenoic acid and three other unidentified metabolites varied slightly due to gene modification.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemistry & molecular biology ; plant sciences
研究领域[WOS]	biochemistry & molecular biology ; plant sciences
关键词[WOS]	flight mass-spectrometry ; maize grain ; nontransgenic counterpart ; bacillus-thuringiensis ; safety evaluation ; oryza-sativa ; plants ; performance ; environment ; expression
收录类别	SCI
语种	英语
WOS记录号	WOS:000302252300012
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/138029]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Chang, Yuwei,Zhao, Chunxia,Zhu, Zhen,et al. Metabolic profiling based on LC/MS to evaluate unintended effects of transgenic rice with cry1Ac and sck genes[J]. plant molecular biology,2012,78(4-5):477-487.
APA	Chang, Yuwei.,Zhao, Chunxia.,Zhu, Zhen.,Wu, Zeming.,Zhou, Jia.,...&Xu, Guowang.(2012).Metabolic profiling based on LC/MS to evaluate unintended effects of transgenic rice with cry1Ac and sck genes.plant molecular biology,78(4-5),477-487.
MLA	Chang, Yuwei,et al."Metabolic profiling based on LC/MS to evaluate unintended effects of transgenic rice with cry1Ac and sck genes".plant molecular biology 78.4-5(2012):477-487.