Monolithic Capillary Column Based Glycoproteomic Reactor for High-Sensitive Analysis of N-Glycoproteome

	Monolithic Capillary Column Based Glycoproteomic Reactor for High-Sensitive Analysis of N-Glycoproteome
	Liu, Jing 1,2; Wang, Fangjun 1; Lin, Hui 1,2; Zhu, Jun 1,2; Bian, Yangyang 1,2; Cheng, Kai 1,2; Zou, Hanfa 1
刊名	analytical chemistry
	2013-03-05
卷号	85 期号:5 页码:2847-2852
英文摘要	despite the importance of protein n-glycosylation in a series of biological processes, in-depth characterization of protein glycosylation is still a challenge due to the high complexity of biological samples and the lacking of highly sensitive detection technologies. we developed a monolithic capillary column based glycoproteomic reactor enabling high. sensitive mapping of n-glycosylation sites from minute amounts of sample. unlike the conventional proteomic reactors with only strong-cation exchange or hydrophilic-interaction chromatography columns, this novel glycoproteomic reactor was composed of an 8 cm long c12 hydrophobic monolithic capillary column for protein digestion and a 6 cm long organic-silica hybrid hydrophilic monolithic capillary column for glycopeptides enrichment and deglycosylation, which could complete whole-sample preparation including protein purification/desalting, tryptic digestion, enrichment, and deglycosylation of glycopeptides within about 3 h. the developed reactor exhibited high detection sensitivity in mapping of n-glycosylation sites by detection limit of horseradish peroxidase as low as 2.5 fmol. this reactor also demonstrated the ability in complex sample analysis, and in total, 486 unique n-glycosylation sites were reliably mapped in three replicate analyses of a protein sample extracted from similar to 10(4) hela cells.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	altered glycosylation ; mass-spectrometry ; proteins ; reveals ; cells
收录类别	SCI
语种	英语
WOS记录号	WOS:000317031600043
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/137787]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jing,Wang, Fangjun,Lin, Hui,et al. Monolithic Capillary Column Based Glycoproteomic Reactor for High-Sensitive Analysis of N-Glycoproteome[J]. analytical chemistry,2013,85(5):2847-2852.
APA	Liu, Jing.,Wang, Fangjun.,Lin, Hui.,Zhu, Jun.,Bian, Yangyang.,...&Zou, Hanfa.(2013).Monolithic Capillary Column Based Glycoproteomic Reactor for High-Sensitive Analysis of N-Glycoproteome.analytical chemistry,85(5),2847-2852.
MLA	Liu, Jing,et al."Monolithic Capillary Column Based Glycoproteomic Reactor for High-Sensitive Analysis of N-Glycoproteome".analytical chemistry 85.5(2013):2847-2852.