Hydrophilic interaction/cation-exchange chromatography for glycopeptide enrichment by using a modified strong-cation exchange material

	Hydrophilic interaction/cation-exchange chromatography for glycopeptide enrichment by using a modified strong-cation exchange material
	Chen, Linlin 1; Dong, Xuefang 2; Cao, Liwei 2; Guo, Zhimou 2; Yu, Long 2; Zou, Lijuan 1; Liang, Xinmiao 2
刊名	analytical methods
	2013
卷号	5 期号:24 页码:6919-6924
英文摘要	protein glycosylation analysis based on mass spectrometry (ms) remains a major analytical challenge, because of the low proportions of glycopeptides in the glycoprotein digest and the suppression effect of the coexisting non-glycosylated peptides. therefore, selective enrichment of glycopeptides prior to ms is of great significance. in this study, we presented hydrophilic interaction/cation-exchange chromatography (hilic/cex) for glycopeptide enrichment by applying a customized material named mex. mex is a silica-based material bonded with two types of functional groups, benzenesulfonic acid and cyano-propyl groups. compared with traditional strong-cation exchange (scx) columns, moderate retention of analytes can be obtained on mex due to the decreased ion-exchange capacity caused by the introduction of the cyano-propyl groups. meanwhile, superior hilic selectivity was also exhibited on mex. in glycosylation analysis, mex exhibited high selectivity and broad coverage for glycopeptides, which indicated the promising potential of the mex material in glycosylation analysis.
WOS标题词	science & technology ; physical sciences ; life sciences & biomedicine ; technology
类目[WOS]	chemistry, analytical ; food science & technology ; spectroscopy
研究领域[WOS]	chemistry ; food science & technology ; spectroscopy
关键词[WOS]	phase liquid-chromatography ; mass-spectrometry ; n-glycans ; glycosylation heterogeneity ; selective enrichment ; cancer-patients ; glycoproteins ; identification ; separation ; proteins
收录类别	SCI
语种	英语
WOS记录号	WOS:000327506600006
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/137699]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Dalian Med Univ, Affiliated Hosp 2, Dept Radiol, Dalian 116044, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Chen, Linlin,Dong, Xuefang,Cao, Liwei,et al. Hydrophilic interaction/cation-exchange chromatography for glycopeptide enrichment by using a modified strong-cation exchange material[J]. analytical methods,2013,5(24):6919-6924.
APA	Chen, Linlin.,Dong, Xuefang.,Cao, Liwei.,Guo, Zhimou.,Yu, Long.,...&Liang, Xinmiao.(2013).Hydrophilic interaction/cation-exchange chromatography for glycopeptide enrichment by using a modified strong-cation exchange material.analytical methods,5(24),6919-6924.
MLA	Chen, Linlin,et al."Hydrophilic interaction/cation-exchange chromatography for glycopeptide enrichment by using a modified strong-cation exchange material".analytical methods 5.24(2013):6919-6924.