Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of MCAT from Synechocystis sp PCC 6803

	Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of MCAT from Synechocystis sp PCC 6803
	Liu, Yinghui 1; Zhang, Yanming 2; Cao, Xupeng 1; Xue, Song 1
刊名	acta crystallographica section f-structural biology and crystallization communications
	2013-11-01
卷号	69 页码:1256-1259
英文摘要	malonyl-coenzymea:acyl-carrier protein transacylase (mcat), which catalyzes the transfer of the malonyl group from malonyl-coa to acyl-carrier protein (acp), is an essential enzyme in type ii fatty-acid synthesis. the enzyme mcat from synechocystis sp. pcc 6803 (spmcat), the first mcat counterpart from a cyanobacterium, was cloned, purified and crystallized in order to determine its three-dimensional crystal structure. a higher-quality crystal with better diffraction was obtained by crystallization optimization. the crystal diffracted to 1.8 angstrom resolution and belonged to the orthorhombic space group p2(1)2(1)2, with unit-cell parameters a = 43.22, b = 149.21, c = 40.59 angstrom. matthews coefficient calculations indicated that the crystal contained one spmcat molecule in the asymmetric unit with a matthews coefficient of 2.18 angstrom(3) da(-1) and a solvent content of 43.65%.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; biochemistry & molecular biology ; biophysics ; crystallography
研究领域[WOS]	biochemistry & molecular biology ; biophysics ; crystallography
关键词[WOS]	acyl carrier protein ; crystal-structure ; malonyl-coa ; mycobacterium-tuberculosis ; transacylase ; models ; site
收录类别	SCI
语种	英语
WOS记录号	WOS:000326569300017
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/137655]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China 2.Agape Struct Solut, Surrey, BC V3W 0E9, Canada
推荐引用方式 GB/T 7714	Liu, Yinghui,Zhang, Yanming,Cao, Xupeng,et al. Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of MCAT from Synechocystis sp PCC 6803[J]. acta crystallographica section f-structural biology and crystallization communications,2013,69:1256-1259.
APA	Liu, Yinghui,Zhang, Yanming,Cao, Xupeng,&Xue, Song.(2013).Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of MCAT from Synechocystis sp PCC 6803.acta crystallographica section f-structural biology and crystallization communications,69,1256-1259.
MLA	Liu, Yinghui,et al."Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of MCAT from Synechocystis sp PCC 6803".acta crystallographica section f-structural biology and crystallization communications 69(2013):1256-1259.