Fast Equilibrium Micro-Extraction from Biological Fluids with Biocompatible Core-Sheath Electrospun Nanofibers

	Fast Equilibrium Micro-Extraction from Biological Fluids with Biocompatible Core-Sheath Electrospun Nanofibers
	Wu, Qian 1,2; Wu, Dapeng 1; Guan, Yafeng 1
刊名	analytical chemistry
	2013-06-18
卷号	85 期号:12 页码:5924-5932
英文摘要	sample preparation methods with high temporal resolution and matrix resistance will benefit fast direct analysis of analytes in a complex matrix, such as drug monitoring in biofluids. in this work, the core-sheath biocompatible electrospun nanofiber was fabricated as a micro-solid phase extraction material. with the poly(n-isopropylacrylamide) (pnipaam) as sheath polymer and polystyrene (ps) as core polymer, the fiber membrane was highly hydrophilic and exhibited good antifouling ability to proteins and cells. its complete expansion in aqueous solution and its nanoscale fiber (100-200 nm) structure offered high mass transfer rate of analytes between liquid and solid phases. the equilibration time of microextraction with this membrane was all shorter than 2 min for eight drugs tested, and the linear ranges covered more than 3 orders of magnitude for most of them. this membrane could be applied to monitor free drugs in plasma and their protein binding kinetics by equilibrium-microextraction with a 2 min temporal resolution. the results showed that the core-sheath electrospun nanofiber membrane would be a better alternative of solid phase material for microextraction with good matrix-resistance ability and high temporal resolution.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	solid-phase microextraction ; plasma-protein binding ; liquid-chromatography ; polymer nanofibers ; wettability ; adsorption ; samples ; fiber ; poly(n-isopropylacrylamide) ; surfaces
收录类别	SCI
语种	英语
WOS记录号	WOS:000320749200044
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/137630]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dept Instrumentat & Analyt Chem, Key Lab Separat Sci Analyt Chem CAS, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wu, Qian,Wu, Dapeng,Guan, Yafeng. Fast Equilibrium Micro-Extraction from Biological Fluids with Biocompatible Core-Sheath Electrospun Nanofibers[J]. analytical chemistry,2013,85(12):5924-5932.
APA	Wu, Qian,Wu, Dapeng,&Guan, Yafeng.(2013).Fast Equilibrium Micro-Extraction from Biological Fluids with Biocompatible Core-Sheath Electrospun Nanofibers.analytical chemistry,85(12),5924-5932.
MLA	Wu, Qian,et al."Fast Equilibrium Micro-Extraction from Biological Fluids with Biocompatible Core-Sheath Electrospun Nanofibers".analytical chemistry 85.12(2013):5924-5932.