Site-Specific Detection of Free Radicals in Membranes Using an Amphiphilic Spin Trap

CORC > 化学研究所 > 中国科学院化学研究所 > 分子动态与稳态结构实验室

	Site-Specific Detection of Free Radicals in Membranes Using an Amphiphilic Spin Trap
	Huang, Saipeng 1,2; Chen, Zhao 3; Du, Libo 1,2; Tian, Qiu 1,2; Liu, Yangping 4; Zheng, Yuansuo 3; Liu, Yang 1
刊名	APPLIED MAGNETIC RESONANCE
	2015-05-01
卷号	46 期号:5 页码:489-504
英文摘要	The detection of free radicals and related species has attracted considerable attention in recent years due to their critical roles in physiological and pathological processes. Among the various methods for the detection of free radicals, electron spin resonance coupled with spin-trapping technique has been an effective approach for the characterization and quantification of free radicals due to its high specificity. In this study, we designed and synthesized a novel amphiphilic spin trap, 2-(diethoxyphosphoryl)-2-heptadecanyl-3,4-dihydro-2H-pyrrole- 1-oxide (DEPHdPO), from 5-(diethoxyphosphoryl)-5-methyl- 1 -pyrroline-N-oxide with a long hydrocarbon chain at the C-5 position of the pyrroline ring, providing the amphiphilic character. The free-radical-trapping ability of DEPHdPO was evaluated by capturing hydroxyl radicals ((OH)-O-center dot), superoxide anions (O-2(center dot-)), and carbon-centered free radicals in a model membrane prepared from sodium dodecyl sulfate (SDS). The results indicate that the hydrophobic hydrocarbon chain of DEPHdPO can be inserted into the inner core of SDS micelles, and the hydrophilic nitronyl functional moiety is located on the surface layer. Thus, various free radicals, including (OH)-O-center dot radicals, O-2(center dot-) anions, and carbon-centered radicals could be site-specifically detected near the membrane surface. Moreover, DEPHdPO could be successfully located on the surface of thylakoid membranes, and the nearby photo-initiated O-2(center dot-) anions could be trapped site-specifically.
收录类别	SCI
语种	英语
公开日期	2015-11-03
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/28617]
专题	化学研究所_分子动态与稳态结构实验室
作者单位	1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Xi An Jiao Tong Univ, Sch Sci, Xian 710049, Peoples R China 4.Tianjin Med Univ, Sch Pharm, Tianjin 300071, Peoples R China
推荐引用方式 GB/T 7714	Huang, Saipeng,Chen, Zhao,Du, Libo,et al. Site-Specific Detection of Free Radicals in Membranes Using an Amphiphilic Spin Trap[J]. APPLIED MAGNETIC RESONANCE,2015,46(5):489-504.
APA	Huang, Saipeng.,Chen, Zhao.,Du, Libo.,Tian, Qiu.,Liu, Yangping.,...&Liu, Yang.(2015).Site-Specific Detection of Free Radicals in Membranes Using an Amphiphilic Spin Trap.APPLIED MAGNETIC RESONANCE,46(5),489-504.
MLA	Huang, Saipeng,et al."Site-Specific Detection of Free Radicals in Membranes Using an Amphiphilic Spin Trap".APPLIED MAGNETIC RESONANCE 46.5(2015):489-504.