Direct production of XYDMY- sex reversal female medaka (Oryzias latipes) by embryo microinjection of TALENs

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	Direct production of XYDMY- sex reversal female medaka (Oryzias latipes) by embryo microinjection of TALENs
	Luo, Daji 1,2; Liu, Yun 3; Chen, Ji 2; Xia, Xiaoqin 2; Cao, Mengxi 2; Cheng, Bin 1; Wang, Xuejuan 1; Gong, Wuming 4; Qiu, Chao 1; Zhang, Yunsheng 2
刊名	SCIENTIFIC REPORTS
	2015-09-14
卷号	5
ISSN号	2045-2322
英文摘要	Medaka is an ideal model for sex determination and sex reversal, such as XY phenotypically female patients in humans. Here, we assembled improved TALENs targeting the DMY gene and generated XYDMY- mutants to investigate gonadal dysgenesis in medaka. DMY-TALENs resulted in indel mutations at the targeted loci (46.8%). DMY-nanos3UTR-TALENs induced mutations were passed through the germline to F1 generation with efficiencies of up to 91.7%. XYDMY- mutants developed into females, laid eggs, and stably passed the YDMY- chromosome to next generation. RNA-seq generated 157 million raw reads from WT male (WT_M_TE), WT female (WT_F_OV) and XYDMY- female medaka (TA_F_OV) gonad libraries. Differential expression analysis identified 144 up-and 293 down-regulated genes in TA_F_OV compared with WT_F_OV, 387 up-and 338 down-regulated genes in TA_F_OV compared with WT_ M_ TE. According to genes annotation and functional prediction, such as Wnt1 and PRCK, it revealed that incomplete ovarian function and reduced fertility of XYDMY- mutant is closely related to the wnt signaling pathway. Our results provided the transcriptional profiles of XYDMY- mutants, revealed the mechanism between sex reversal and DMY in medaka, and suggested that XYDMY- medaka was a novel mutant that is useful for investigating gonadal dysgenesis in phenotypic female patients with the 46, XY karyotype.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	TARGETED GENE DISRUPTION ; EFFECTOR NUCLEASES ; DETERMINING REGION ; PHENOTYPIC FEMALE ; CLEAVAGE DOMAIN ; Y-CHROMOSOME ; FISH ; ZEBRAFISH ; DMRT1 ; DMY
收录类别	SCI
语种	英语
WOS记录号	WOS:000361149600001
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/27190]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430071, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 3.Chinese Univ Hong Kong, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China 4.Univ Minnesota, Lillehei Heart Inst, Minneapolis, MN USA
推荐引用方式 GB/T 7714	Luo, Daji,Liu, Yun,Chen, Ji,et al. Direct production of XYDMY- sex reversal female medaka (Oryzias latipes) by embryo microinjection of TALENs[J]. SCIENTIFIC REPORTS,2015,5.
APA	Luo, Daji.,Liu, Yun.,Chen, Ji.,Xia, Xiaoqin.,Cao, Mengxi.,...&Hu, Wei.(2015).Direct production of XYDMY- sex reversal female medaka (Oryzias latipes) by embryo microinjection of TALENs.SCIENTIFIC REPORTS,5.
MLA	Luo, Daji,et al."Direct production of XYDMY- sex reversal female medaka (Oryzias latipes) by embryo microinjection of TALENs".SCIENTIFIC REPORTS 5(2015).