Inhibition of gamma-secretase by retinoic acid chalcone (RAC) induces G2/M arrest and triggers apoptosis in renal cell carcinoma

	Inhibition of gamma-secretase by retinoic acid chalcone (RAC) induces G2/M arrest and triggers apoptosis in renal cell carcinoma
	Li, Qing-Chun 1; Li, Hong-Jun 2; Liu, Shi 3; Liang, Yun 2; Wang, Xue 2; Cui, Lei 4
刊名	INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
	2015
卷号	8 期号:3 页码:2400-2407
关键词	Renal cell carcinoma apoptosis Notch1 Jagged1
英文摘要	The present study was devised to investigate the effect of RAC on inhibition of cell proliferation and apoptosis of renal carcinoma cells. MTT assay and flow cytometry analysis were used to determine cell proliferation and apoptosis along with cell cycle examination. Western blot analysis and immunohistochemistry were used for the detection of expression levels of Notch1 and Jagged1 in renal cell carcinoma (RCC) and normal kidney tissues. The results revealed a significant inhibition of cell proliferation, G2/M phase cell cycle arrest and cell apoptosis at 30 mu M concentration of RAC after 72 h. In ACHN and 769-P cells, the population in G2/M phase was increased to 45.27, and 54.23% respectively on treatment with 30 mu M RAC for 72 h. In 769-P and ACHN renal carcinoma cells treatment with 30 mu M RAC caused 69.71 and 59.27% of the cells to undergo apoptosis compared to 5.23 and 4.93% respectively in control cells. The positive staining rates of Notch1 and Jagged1 in renal carcinoma tissues were 95.3 and 93.0% compared to normal kidney tissues 36.4 and 42.4% respectively. Treatment of renal carcinoma tissues caused a significant decrease in staining rates of Notch1 and Jagged1 after 96 h. Thus RAC can be a potent agent in the treatment of renal cell carcinoma.
收录类别	SCI
语种	英语
公开日期	2015-11-02
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/28134]
专题	化学研究所_高分子物理与化学实验室
作者单位	1.Jilin Univ, China Japan Union Hosp, Dept Gastrointestinal Surg, Changchun 130033, Jilin, Peoples R China 2.Jilin Univ, China Japan Union Hosp, Ctr Phys Examinat, Changchun 130033, Jilin, Peoples R China 3.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Jilin, Peoples R China 4.Jilin Inst Drug Control, Changchun 130033, Jilin, Peoples R China
推荐引用方式 GB/T 7714	Li, Qing-Chun,Li, Hong-Jun,Liu, Shi,et al. Inhibition of gamma-secretase by retinoic acid chalcone (RAC) induces G2/M arrest and triggers apoptosis in renal cell carcinoma[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,2015,8(3):2400-2407.
APA	Li, Qing-Chun,Li, Hong-Jun,Liu, Shi,Liang, Yun,Wang, Xue,&Cui, Lei.(2015).Inhibition of gamma-secretase by retinoic acid chalcone (RAC) induces G2/M arrest and triggers apoptosis in renal cell carcinoma.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,8(3),2400-2407.
MLA	Li, Qing-Chun,et al."Inhibition of gamma-secretase by retinoic acid chalcone (RAC) induces G2/M arrest and triggers apoptosis in renal cell carcinoma".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 8.3(2015):2400-2407.