Gold Nanoparticle-Based Dynamic Light Scattering Assay for Mercury Ion Detection

	Gold Nanoparticle-Based Dynamic Light Scattering Assay for Mercury Ion Detection
	Ma Li-Na ; Liu Dian-Jun ; Wang Zhen-Xin
刊名	分析化学 chinese journal of analytical chemistry
	2014
卷号	42 期号:3 页码:332-336
关键词	LABEL-FREE PROBES APTAMER BINDING
通讯作者	wang,zx
英文摘要	a gold nanoparticle (gnp)-based dynamic light scattering (dls) assay has been developed for detecting mercury ion (hg2+) in aqueous solution. the gnps were able to maintain monodisperse state in relative high ionic strength (0.1 m nacl) aqueous solution because hg2+ aptamer 5'-tttcttctttcttccccccttgtttgttgttt-3' (probe dna) could adsorb on the gnp surface by electrostatic interaction. in the presence of hg2+, the probe dna molecules were easily desorbed from gnp surface because of t-hg2+-t formation. the unprotected gnps form aggregates in 100 mm nacl solution, resulting in increased significantly the average hydrodynamic diameter of gnps. under the conditions of ph 7.43, 110 nm aptamer, 100 mm nacl and 30 min incubation time of hg2+ with aptamer, the difference of average hydrodynamic diameter (delta d) of gnps was increased linearly with logarithm of hg2+ concentration over the range from 0.1 nm to 5 mu m, with a detection limit of 0.1 nm. moreover, satisfactory results were obtained when the proposed method was applied to detect hg2+ in real samples.
收录类别	SCI
语种	英语
WOS记录号	WOS:000334581800004
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/57547]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Ma Li-Na,Liu Dian-Jun,Wang Zhen-Xin. Gold Nanoparticle-Based Dynamic Light Scattering Assay for Mercury Ion Detection[J]. 分析化学 chinese journal of analytical chemistry,2014,42(3):332-336.
APA	Ma Li-Na,Liu Dian-Jun,&Wang Zhen-Xin.(2014).Gold Nanoparticle-Based Dynamic Light Scattering Assay for Mercury Ion Detection.分析化学 chinese journal of analytical chemistry,42(3),332-336.
MLA	Ma Li-Na,et al."Gold Nanoparticle-Based Dynamic Light Scattering Assay for Mercury Ion Detection".分析化学 chinese journal of analytical chemistry 42.3(2014):332-336.