Single-molecule-force spectroscopy study of the mechanism of interactions between TSP-1 and CD47

	Single-molecule-force spectroscopy study of the mechanism of interactions between TSP-1 and CD47
	Pan YanGang ; Wang Feng ; Liu YanHou
刊名	中国科学:化学 science china-chemistry
	2014
卷号	57 期号:12 页码:1716-1722
关键词	INTEGRIN-ASSOCIATED PROTEIN CELL-MEMBRANES FC-GAMMA MICROSCOPY RECEPTOR THROMBOSPONDIN-1 PHAGOCYTOSIS ANGIOGENESIS RECOGNITION INHIBITOR
通讯作者	yang,yg
英文摘要	the 4n1k peptide, which is derived from the c-terminal domain of thrombospondin-1 (tsp-1), is usually used as a functional mimic peptide for tsp-1. knowledge about the interaction force of 4n1k/cd47 is important in explaining how tsp-1 affects the biological effect of cd47. here we used a single-molecule force spectroscopy (smfs) technique to explore the interaction of 4n1k/cd47 on both normal and oxidative human red blood cells (hrbcs) at single-molecule level. there was no interaction force between 4n1k and cd47 on normal hrbcs; however, we did find 4n1k-bound cd47 on oxidative hrbcs. we also detected interaction forces for 4n1k/cd47(ex) (extracellular domain of human cd47), and 4n1k/oxidative cd47(ex). the interaction forces of 4n1k/cd47(ex) were almost consistent with those of 4n1k/oxidative cd47(ex) at the same loading rate. these results suggest that the conformational change of cd47 is critical for 4n1k-cd47 interaction on oxidative hrbcs.
收录类别	SCI
语种	英语
公开日期	2015-10-27
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/57500]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Pan YanGang,Wang Feng,Liu YanHou. Single-molecule-force spectroscopy study of the mechanism of interactions between TSP-1 and CD47[J]. 中国科学:化学 science china-chemistry,2014,57(12):1716-1722.
APA	Pan YanGang,Wang Feng,&Liu YanHou.(2014).Single-molecule-force spectroscopy study of the mechanism of interactions between TSP-1 and CD47.中国科学:化学 science china-chemistry,57(12),1716-1722.
MLA	Pan YanGang,et al."Single-molecule-force spectroscopy study of the mechanism of interactions between TSP-1 and CD47".中国科学:化学 science china-chemistry 57.12(2014):1716-1722.