Ultrafiltration LC-PDA-ESI/MS combined with reverse phase-medium pressure liquid chromatography for screening and isolation potential alpha-glucosidase inhibitors from Scutellaria baicalensis Georgi

	Ultrafiltration LC-PDA-ESI/MS combined with reverse phase-medium pressure liquid chromatography for screening and isolation potential alpha-glucosidase inhibitors from Scutellaria baicalensis Georgi
	Wang, Jing ; Liu, Shu ; Li, Sainan ; Song,Fengrui ; Zhang,Yuchi ; Liu,Zhiqiang ; Liu,Chun-ming
刊名	analytical methods
	2014
卷号	6 期号:15 页码:5918-5924
关键词	MASS-SPECTROMETRY FLAVONOIDS HPLC MS PHARMACOKINETICS METABOLOMICS LEAVES
通讯作者	liu,s
英文摘要	diabetes mellitus (dm) has become a worldwide health problem alpha-glucosidase inhibitors are widely used in the treatment of type 2 diabetes, and natural products are considered as the promising sources of alpha-glucosidase inhibitors. in this research, ultrafiltration liquid chromatography with photodiode array detection coupled to electrospray ionization tandem mass spectrometry (ultrafiltration lc-pda-esi/ms) were applied to screen and identify alpha-glucosidase inhibitors from scutellaria baicalensis. following this, reverse-phase medium-pressure liquid chromatography (rp-mplc) was applied to separate and isolate the active constituents. as a result, eleven major compounds in s. baicalensis were screened and identified as alpha-glucosidase inhibitors by ultrafiltration lc-pda-esi/ms and six ligands, baicalin, wogonoside, 5,7,3,2',6'-pentahydroxy flavanone, chrysin-6-c-arabinosyl-8-c-glucoside, chrysin-6-c-glucosyl-8-c-arabinoside and wogonin, were isolated by rp-mplc. the purities of the six compounds were 89.7%, 83.5%, 76.2%, 93.7%, 75.1% and 96.2%, respectively, as determined by high performance liquid chromatography. the results demonstrate that the method for systematic isolation of bioactive components in s. baicalensis guided by ultrafiltration lc-pda-esi/ms is a feasible and efficient technique and could be expected to extend for the separation of other enzyme inhibitors.
收录类别	SCI
语种	英语
公开日期	2015-10-27
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/57478]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang, Jing,Liu, Shu,Li, Sainan,et al. Ultrafiltration LC-PDA-ESI/MS combined with reverse phase-medium pressure liquid chromatography for screening and isolation potential alpha-glucosidase inhibitors from Scutellaria baicalensis Georgi[J]. analytical methods,2014,6(15):5918-5924.
APA	Wang, Jing.,Liu, Shu.,Li, Sainan.,Song,Fengrui.,Zhang,Yuchi.,...&Liu,Chun-ming.(2014).Ultrafiltration LC-PDA-ESI/MS combined with reverse phase-medium pressure liquid chromatography for screening and isolation potential alpha-glucosidase inhibitors from Scutellaria baicalensis Georgi.analytical methods,6(15),5918-5924.
MLA	Wang, Jing,et al."Ultrafiltration LC-PDA-ESI/MS combined with reverse phase-medium pressure liquid chromatography for screening and isolation potential alpha-glucosidase inhibitors from Scutellaria baicalensis Georgi".analytical methods 6.15(2014):5918-5924.