Novel core-shell Cerium(IV)-immobilized magnetic polymeric microspheres for selective enrichment and rapid separation of phosphopeptides

	Novel core-shell Cerium(IV)-immobilized magnetic polymeric microspheres for selective enrichment and rapid separation of phosphopeptides
	Wang, Zhi-Gang ; Cheng, Gong ; Liu, Yan-Lin ; Zhang,Ji-Lin ; Sun,De-Hui ; Ni,Jia-Zuan
刊名	journal of colloid and interface science
	2014
卷号	417 期号:1 页码:217-226
关键词	TOF MS ANALYSIS PHOSPHORYLATED BIOMOLECULES LABELING PHOSPHOPEPTIDES PHOSPHOPROTEOME ANALYSIS AFFINITY MICROSPHERES MASS-SPECTROMETRY NANOPARTICLES MALDI CAPTURE PROTEINS
通讯作者	zhang,jl
英文摘要	in this work, novel magnetic polymeric core-shell structured microspheres with immobilized ce(iv), fe3o4@sio2@pvpa-ce(iv), were designed rationally and synthesized successfully via a facile route for the first time. magnetic fe3o4@sio2 microspheres were first prepared by directly coating a thin layer of silica onto fe3o4 magnetic particles using a sol-gel method, a poly(vinylphosphonic acid) (pvpa) shell was then coated on the fe3o4@sio2 microspheres to form fe3o4@sio2@pvpa microspheres through a radical polymerization reaction, and finally ce(iv) ions were robustly immobilized onto the fe3o4@sio2@pvpa microspheres through strong chelation between ce(iv) ions and phosphate moieties in the pvpa. the applicability of the fe3o4@sio2@pvpa-ce(iv) microspheres for selective enrichment and rapid separation of phosphopeptides from proteolytic digests of standard and real protein samples was investigated. the results demonstrated that the core-shell structured fe3o4@sio2@pvpa-ce(iv) microspheres with abundant ce(iv) affinity sites and excellent magnetic responsiveness can effectively purify phosphopeptides from complex biosamples for ms detection taking advantage of the rapid magnetic separation and the selective affinity between ce(iv) ions and phosphate moieties of the phosphopeptides. furthermore, they can be effectively recycled and show good reusability, and have better performance than commercial tio2 beads and homemade fe3o4@pmaa-ce(iv) microspheres. thus the fe3o4@sio2@pvpa-ce(iv) microspheres can benefit greatly the mass spectrometric qualitative analysis of phosphopeptides in phosphoproteome research. (c) 2013 elsevier inc. all rights reserved.
收录类别	SCI
语种	英语
公开日期	2015-10-27
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/57334]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang, Zhi-Gang,Cheng, Gong,Liu, Yan-Lin,et al. Novel core-shell Cerium(IV)-immobilized magnetic polymeric microspheres for selective enrichment and rapid separation of phosphopeptides[J]. journal of colloid and interface science,2014,417(1):217-226.
APA	Wang, Zhi-Gang,Cheng, Gong,Liu, Yan-Lin,Zhang,Ji-Lin,Sun,De-Hui,&Ni,Jia-Zuan.(2014).Novel core-shell Cerium(IV)-immobilized magnetic polymeric microspheres for selective enrichment and rapid separation of phosphopeptides.journal of colloid and interface science,417(1),217-226.
MLA	Wang, Zhi-Gang,et al."Novel core-shell Cerium(IV)-immobilized magnetic polymeric microspheres for selective enrichment and rapid separation of phosphopeptides".journal of colloid and interface science 417.1(2014):217-226.