Metabonomic Characterization of The Low-grade Human Astrocytomas and Meningiomas Using Magic-angle Spinning H-1 Nuclear Magnetic Resonance Spectroscopy and Principal Component Analysis

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 2011年以前论文发表（包括2011年）

	Metabonomic Characterization of The Low-grade Human Astrocytomas and Meningiomas Using Magic-angle Spinning H-1 Nuclear Magnetic Resonance Spectroscopy and Principal Component Analysis
	Chen Wen-Xue 1,2; Lou Hai-Yan 3,4; Zhang Hong-Ping 5; Nie Xiu 6; Xiang Yun 1,2; Yang Yong-Xia 1,2; Wu Guang-Yao 1,5; Qi Jian-Pin 3; Yue Yong 1; Lei Hao 1
刊名	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
	2008-10-01
卷号	35 期号:10 页码:1142-1153
关键词	human brain tumor glioma astrocytomas meningioma high resolution magic-angle spinning (HRMAS) nuclear magnetic resonance spectroscopy pattern recognition statistical analysis
英文摘要	Metabolic characteristics of 39 human brain tumor tissues, including 15 astrocytomas, 13 fibroblastic meningiomas and 11 transitional meningiomas from 39 individual patients, have been studied using high resolution magic-angle spinning (HRMAS) H-1 NMR spectroscopy in conjunction with principal component analysis (PCA). With rich metabolite information, H-1 NMR spectra showed that the tumor-tissue metabonome was dominated by lipids, lactate, myo-inositol, creatine, choline metabolites such as choline, phosphocholine and glycerophosphocholine, amino acids such as alanine, glutamate, glutamine, taurine, N-acetyl-aspartate and glutathione. PCA of the tumor NMR spectra clearly showed metabonomic differences between low-grade astrocytomas and meningiomas whereas such differences were more moderate between fibroblastic and transitional meningiomas. Compared with meningiomas, the low-grade astrocytomas had higher levels of glycerophosphocholine, phosphocholine, myo-inositol and creatine but lower levels of alanine, glutamate, glutamine, glutathione and taurine. The N-acetyl-aspartate level was low but detectable in low-grade astrocytomas whereas it was not detectable in meningiomas. It is concluded that tissue metabonomics technology consisting of HRMAS H-1 NMR spectroscopy and multivariate data analysis (MVDA) offers a useful tool (1) for distinguishing different types of brain tumors, (2) for providing the metabolic information for human brain tumors, which are potentially useful for understanding biochemistry of tumor progression.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	HUMAN BRAIN-TUMORS ; PROTON MR SPECTROSCOPY ; QUANTITATIVE IN-VIVO ; SHORT-ECHO-TIME ; HUMAN CEREBRAL NEOPLASMS ; HIGH-RESOLUTION H-1-NMR ; TEMPERATURE CALIBRATION ; METABOLIC PROFILES ; TISSUE SAMPLES ; RAT-BRAIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000260384000007
公开日期	2015-10-13
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/8107]
专题	武汉物理与数学研究所_2011年以前论文发表（包括2011年）
作者单位	1.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Huazhong Univ Sci & Technol, Tongji Med Univ, Tongji Hosp, Wuhan 430022, Peoples R China 4.Zhejiang Univ, Coll Med, Affiliated Hosp 1, Hangzhou 310003, Zhejiang, Peoples R China 5.Wuhan Univ, Coll Med, Zhongnan Hosp, Wuhan 430071, Peoples R China 6.Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Wuhan 430022, Peoples R China
推荐引用方式 GB/T 7714	Chen Wen-Xue,Lou Hai-Yan,Zhang Hong-Ping,et al. Metabonomic Characterization of The Low-grade Human Astrocytomas and Meningiomas Using Magic-angle Spinning H-1 Nuclear Magnetic Resonance Spectroscopy and Principal Component Analysis[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2008,35(10):1142-1153.
APA	Chen Wen-Xue.,Lou Hai-Yan.,Zhang Hong-Ping.,Nie Xiu.,Xiang Yun.,...&Deng Feng.(2008).Metabonomic Characterization of The Low-grade Human Astrocytomas and Meningiomas Using Magic-angle Spinning H-1 Nuclear Magnetic Resonance Spectroscopy and Principal Component Analysis.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,35(10),1142-1153.
MLA	Chen Wen-Xue,et al."Metabonomic Characterization of The Low-grade Human Astrocytomas and Meningiomas Using Magic-angle Spinning H-1 Nuclear Magnetic Resonance Spectroscopy and Principal Component Analysis".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 35.10(2008):1142-1153.