Effects of microcystin-LR on gut microflora in different gut regions of mice | |
Chen, Jing1,2; Xie, Ping1; Lin, Juan1,2; He, Jun1; Zeng, Cheng3; Chen, Jun1 | |
刊名 | JOURNAL OF TOXICOLOGICAL SCIENCES
![]() |
2015-08-01 | |
卷号 | 40期号:4页码:485-494 |
关键词 | MC-LR Gut microbial community Mice Intestine |
ISSN号 | 0388-1350 |
英文摘要 | To reveal the toxicological effects of the hepatotoxic microcystin-leucine arginine (MC-LR) on gut microbial community composition in different gut regions, we conducted a subchronic exposure of BALB/c mice to MC-LR via intragastric administration. Denaturing gradient gel electrophoresis (DGGE) was employed to profile the shifts of microbes after MC-LR treatment in the jejuno-ileum, caecum and colon. DGGE profiles analysis showed that MC-LR increased the microbial species richness (number of microbial bands) in the caecum and colon as well as microbial diversity (Shannon-Wiener index) in the caecum. The cluster analysis of DGGE profiles indicated that the microbial structures in the caecum and colon shifted significantly after MC-LR treatment, while that in the jejuno-ileum did not. All the relatively decreased gut microbes belonged to Clostridia in the Firmicutes phylum, and most of them were Lachnospiraceae. The increased ones derived from a variety of microbes including species from Porphyromonadaceae and Prevotellaceae in the Bacteroidetes phylum, as well as Lachnospiraceae and Ruminococcaceae in the Firmicutes phylum, and among which, the increase of Barnesiella in Porphyromonadaceae was most remarkable. In conclusion, subchronic exposure to MC-LR could disturb the balance of gut microbes in mice, and its toxicological effects varied between the jejuno-ileum and the other two gut regions. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Toxicology |
研究领域[WOS] | Toxicology |
关键词[WOS] | SPINNING H-1-NMR SPECTROSCOPY ; FATTY LIVER-DISEASE ; IN-VITRO EVALUATION ; 16S RIBOSOMAL-RNA ; BACTERIAL DIVERSITY ; INTESTINAL MICROBIOTA ; CYANOBACTERIAL TOXINS ; MICROORGANISMS ; HEALTH ; GENES |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000359180600007 |
内容类型 | 期刊论文 |
源URL | [http://ir.ihb.ac.cn/handle/342005/25971] ![]() |
专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
作者单位 | 1.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Jing,Xie, Ping,Lin, Juan,et al. Effects of microcystin-LR on gut microflora in different gut regions of mice[J]. JOURNAL OF TOXICOLOGICAL SCIENCES,2015,40(4):485-494. |
APA | Chen, Jing,Xie, Ping,Lin, Juan,He, Jun,Zeng, Cheng,&Chen, Jun.(2015).Effects of microcystin-LR on gut microflora in different gut regions of mice.JOURNAL OF TOXICOLOGICAL SCIENCES,40(4),485-494. |
MLA | Chen, Jing,et al."Effects of microcystin-LR on gut microflora in different gut regions of mice".JOURNAL OF TOXICOLOGICAL SCIENCES 40.4(2015):485-494. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论