Geometric control of capillary architecture via cell-matrix mechanical interactions

CORC > 自动化研究所 > 中国科学院自动化研究所 > 复杂系统管理与控制国家重点实验室 > 先进控制与自动化团队

	Geometric control of capillary architecture via cell-matrix mechanical interactions
	Sun, Jian 1; Jamilpour, Nima 1; Wang, Fei-Yue2 ; Wong, Pak Kin 1
刊名	BIOMATERIALS
	2014-03-01
卷号	35 期号:10 页码:3273-3280
关键词	Geometric constraints Capillary morphogenesis Cell-matrix interactions Hydrogel Angiogenesis
英文摘要	Capillary morphogenesis is a multistage, multicellular activity that plays a pivotal role in various developmental and pathological situations. In-depth understanding of the regulatory mechanism along with the capability of controlling the morphogenic process will have direct implications on tissue engineering and therapeutic angiogenesis. Extensive research has been devoted to elucidate the biochemical factors that regulate capillary morphogenesis. The roles of geometric confinement and cell-matrix mechanical interactions on the capillary architecture, nevertheless, remain largely unknown. Here, we show geometric control of endothelial network topology by creating physical confinements with microfabricated fences and wells. Decreasing the thickness of the matrix also results in comparable modulation of the network architecture, supporting the boundary effect is mediated mechanically. The regulatory role of cell-matrix mechanical interaction on the network topology is further supported by alternating the matrix stiffness by a cell-inert PEG-dextran hydrogel. Furthermore, reducing the cell traction force with a Rho-associated protein kinase inhibitor diminishes the boundary effect. Computational biomechanical analysis delineates the relationship between geometric confinement and cell-matrix mechanical interaction. Collectively, these results reveal a mechanoregulation scheme of endothelial cells to regulate the capillary network architecture via cell-matrix mechanical interactions. (C) 2014 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Technology
类目[WOS]	Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]	Engineering ; Materials Science
关键词[WOS]	ATOMIC-FORCE MICROSCOPY ; IN-VITRO ; ENDOTHELIAL-CELLS ; TISSUE ; ANGIOGENESIS ; SUBSTRATE ; MORPHOGENESIS ; STIFFNESS ; NETWORKS ; FEEL
收录类别	SCI
语种	英语
WOS记录号	WOS:000332188400014
公开日期	2015-09-22
内容类型	期刊论文
源URL	[http://ir.ia.ac.cn/handle/173211/8026]
专题	自动化研究所_复杂系统管理与控制国家重点实验室_先进控制与自动化团队
作者单位	1.Univ Arizona, Dept Aerosp & Mech Engn, Tucson, AZ 85721 USA 2.Chinese Acad Sci, Inst Automat, Key Lab Complex Syst & Intelligence Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Sun, Jian,Jamilpour, Nima,Wang, Fei-Yue,et al. Geometric control of capillary architecture via cell-matrix mechanical interactions[J]. BIOMATERIALS,2014,35(10):3273-3280.
APA	Sun, Jian,Jamilpour, Nima,Wang, Fei-Yue,&Wong, Pak Kin.(2014).Geometric control of capillary architecture via cell-matrix mechanical interactions.BIOMATERIALS,35(10),3273-3280.
MLA	Sun, Jian,et al."Geometric control of capillary architecture via cell-matrix mechanical interactions".BIOMATERIALS 35.10(2014):3273-3280.