Expression and Characterization of a Novel Thermo-Alkalistable Lipase from Hyperthermophilic Bacterium Thermotoga maritima | |
Tian, Rui1; Chen, Huayou1,2,3; Ni, Zhong1; Zhang, Qing1; Zhang, Zhongge3; Zhang, Tianxi1; Zhang, Chunxia1; Yang, Shengli1 | |
刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2015-07-01 | |
卷号 | 176期号:5页码:1482-1497 |
关键词 | Lipase Thermostable enzyme Thermotoga maritima Alkali resistance Methanol-activated |
ISSN号 | 0273-2289 |
英文摘要 | A gene coding for lipase (Tm1350) from the hyperthermophilic bacterium Thermotoga maritima MSB8 was cloned and overexpressed by Escherichia coli. The enzyme can degrade substrates with both short and long acyl chain lengths. The apparent Km and Vmax values for p-nitrophenyl butyrate were 8 mM and 333 U/mg, respectively. The enzyme displayed optimal activity at pH 7.5 and 70 A degrees C, maintained 66 % of the original activity after 8 h of incubation, and its half-lives at pHs 9 and 10 were 8 and 1 h. The activity of Tm1350 was stimulated up to 131 or 151 % of the original activity by incubating with 4 M urea or 20 % (v/v) methanol, and 90.1 or 70.2 % of the activity was maintained after 8 h incubation of the enzyme in 20 or 75 % (v/v) of the methanol, showing potential for biodiesel production. The activity of the enzyme without cysteine residue was stimulated up to 618 and 550 % of the original activity by incubating with dithiothreitol (DTT) and reduced glutathione (GSH) at a concentration of 1 mM. However, the circular dichroism spectra of the enzyme have no obvious change after DTT treatment. It is speculated that DTT interacts with potential residues in some key active sites without influence of structure. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
关键词[WOS] | HIGH-LEVEL EXPRESSION ; PYROCOCCUS-FURIOSUS ; ORGANIC-SOLVENTS ; PURIFICATION ; ESTERASE ; STRAIN ; ENZYME ; LYSOPHOSPHOLIPASE ; DITHIOTHREITOL ; REAGENT |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000358379400018 |
内容类型 | 期刊论文 |
源URL | [http://ir.ipe.ac.cn/handle/122111/19412] ![]() |
专题 | 过程工程研究所_生化工程国家重点实验室 |
作者单位 | 1.Jiangsu Univ, Inst Life Sci, Zhenjiang 212013, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 10090, Peoples R China 3.Univ Calif San Diego, Div Biol Sci, La Jolla, CA 92093 USA |
推荐引用方式 GB/T 7714 | Tian, Rui,Chen, Huayou,Ni, Zhong,et al. Expression and Characterization of a Novel Thermo-Alkalistable Lipase from Hyperthermophilic Bacterium Thermotoga maritima[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2015,176(5):1482-1497. |
APA | Tian, Rui.,Chen, Huayou.,Ni, Zhong.,Zhang, Qing.,Zhang, Zhongge.,...&Yang, Shengli.(2015).Expression and Characterization of a Novel Thermo-Alkalistable Lipase from Hyperthermophilic Bacterium Thermotoga maritima.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,176(5),1482-1497. |
MLA | Tian, Rui,et al."Expression and Characterization of a Novel Thermo-Alkalistable Lipase from Hyperthermophilic Bacterium Thermotoga maritima".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 176.5(2015):1482-1497. |
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