Mixed mycobacterial infections in farmed sturgeons

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	Mixed mycobacterial infections in farmed sturgeons
	Zhang, De Feng 1,2; Ji, Cheng 3; Zhang, Xu Jie 1; Li, Tong Tong 1; Li, Ai Hua 1; Gong, Xiao Ning 1
刊名	AQUACULTURE RESEARCH
	2015-08-01
卷号	46 期号:8 页码:1914-1923
关键词	sturgeon mixed infection Mycobacterium mycobacteriosis
ISSN号	1355-557X
通讯作者	Li, AH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
英文摘要	Based on microbiological and histopathological examinations and DNA sequencing, several outbreaks of mycobacteriosis in the reared sturgeons, including Chinese sturgeon (Acipenser sinensis Gray) and Amur sturgeon (Acipenser schrencki), were identified during 2009 to 2010. Forty-nine isolates of non-tuberculous mycobacteria(NTM)were isolated from 19 diseased sturgeons. In total, seven species of Mycobacterium were identified, namely, Mycobacterium chelonae, Mycobacterium marinum, Mycobacterium gordonae, Mycobacterium fortuitum, Mycobacterium szulgai, Mycobacterium arupense and Mycobacterium porcinum. Among them, M. marinum was found to be more prevalent (89.5%) compared with the other mycobacterial species. When two molecular biological methods, PCR-DGGE (denaturing gradient gel electrophoresis) analysis and rpoB gene library sequencing, were used to analyse the mycobacterial DNAs extracted from the diseased fish tissues, mixed infections of two or three mycobacterial species were found being the predominant infection form (94.7%) in sturgeon mycobacteriosis. M. marinum was the only one species that caused sturgeon mycobacteriosis alone. Virulence assay showed that M. marinum possessed stronger pathogenicity to zebrafish killing 100% of fish in 28days at 10(3) cfu/fish than the other species. These results suggested that M. marinum is the major pathogenic bacteria in sturgeon mycobacteriosis. To the best of our knowledge, this study is the first report on mycobacteriosis in farmed Chinese and Amur sturgeons as well as the first isolation of M. porcinum and M. arupense from fish.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries
研究领域[WOS]	Fisheries
关键词[WOS]	ORNAMENTAL FISH ; SP-NOV. ; AQUARIUM FISH ; SPP. ; IDENTIFICATION ; INFLAMMATION ; AQUACULTURE ; ARUPENSE ; CULTURE ; LEVEL
收录类别	SCI
语种	英语
WOS记录号	WOS:000357820100012
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/23884]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou, Guangdong, Peoples R China 3.Soochow Univ, Sch Basic Med & Biol Sci, Ctr Circadian Clocks, Suzhou, Peoples R China
推荐引用方式 GB/T 7714	Zhang, De Feng,Ji, Cheng,Zhang, Xu Jie,et al. Mixed mycobacterial infections in farmed sturgeons[J]. AQUACULTURE RESEARCH,2015,46(8):1914-1923.
APA	Zhang, De Feng,Ji, Cheng,Zhang, Xu Jie,Li, Tong Tong,Li, Ai Hua,&Gong, Xiao Ning.(2015).Mixed mycobacterial infections in farmed sturgeons.AQUACULTURE RESEARCH,46(8),1914-1923.
MLA	Zhang, De Feng,et al."Mixed mycobacterial infections in farmed sturgeons".AQUACULTURE RESEARCH 46.8(2015):1914-1923.