Immunotoxicity Assessment of Rice-Derived Recombinant Human Serum Albumin Using Human Peripheral Blood Mononuclear Cells

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	Immunotoxicity Assessment of Rice-Derived Recombinant Human Serum Albumin Using Human Peripheral Blood Mononuclear Cells
	Fu, Kai 1,2; Cheng, Qin 1,2; Liu, Zhenwei 1,2; Chen, Zhen 1,2; Wang, Yan 4; Ruan, Honggang 3; Zhou, Lu 3; Xiong, Jie 3; Xiao, Ruijing 3; Liu, Shengwu 3
刊名	PLOS ONE
	2014-08-06
卷号	9 期号:8
ISSN号	1932-6203
英文摘要	Human serum albumin (HSA) is extensively used in clinics to treat a variety of diseases, such as hypoproteinemia, hemorrhagic shock, serious burn injuries, cirrhotic ascites and fetal erythroblastosis. To address supply shortages and high safety risks from limited human donors, we recently developed recombinant technology to produce HSA from rice endosperm. To assess he risk potential of HSA derived from Oryza sativa (OsrHSA) before a First-in-human (FIH) trial, we compared OsrHSA and plasma-derived HSA (pHSA), evaluating the potential for an immune reaction and toxicity using human peripheral blood mononuclear cells (PBMCs). The results indicated that neither OsrHSA nor pHSA stimulated T cell proliferation at 1x and 5x dosages. We also found no significant differences in the profiles of the CD4(+) and CD8(+) T cell subsets between OsrHSA- and pHSA-treated cells. Furthermore, the results showed that there were no significant differences between OsrHSA and pHSA in the production of cytokines such as interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-10 and IL-4. Our results demonstrated that OsrHSA has equivalent immunotoxicity to pHSA when using the PBMC model. Moreover, this ex vivo system could provide an alternative approach to predict potential risks in novel biopharmaceutical development.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	MONOCLONAL-ANTIBODY TGN1412 ; PROTEIN O-GLYCOSYLATION ; SACCHAROMYCES-CEREVISIAE ; IMMUNE-RESPONSES ; CYTOKINE STORM ; EXPRESSION ; SECRETION ; SAFETY ; YEAST ; PURIFICATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000339995100070
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/23809]
专题	水生生物研究所_分析测试中心_期刊论文
作者单位	1.Wuhan Univ, State Key Lab Hybrid Rice, Wuhan 430072, Peoples R China 2.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China 3.Wuhan Univ, Dept Immunol, Coll Basic Med Sci, Wuhan 430072, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, Anal & Testing Ctr, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Fu, Kai,Cheng, Qin,Liu, Zhenwei,et al. Immunotoxicity Assessment of Rice-Derived Recombinant Human Serum Albumin Using Human Peripheral Blood Mononuclear Cells[J]. PLOS ONE,2014,9(8).
APA	Fu, Kai.,Cheng, Qin.,Liu, Zhenwei.,Chen, Zhen.,Wang, Yan.,...&Yang, Daichang.(2014).Immunotoxicity Assessment of Rice-Derived Recombinant Human Serum Albumin Using Human Peripheral Blood Mononuclear Cells.PLOS ONE,9(8).
MLA	Fu, Kai,et al."Immunotoxicity Assessment of Rice-Derived Recombinant Human Serum Albumin Using Human Peripheral Blood Mononuclear Cells".PLOS ONE 9.8(2014).