Sources of organic matter affect depth-related microbial community composition in sediments of Lake Erhai, Southwest China

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	Sources of organic matter affect depth-related microbial community composition in sediments of Lake Erhai, Southwest China
	Xiong, Wei 1; Xie, Ping 2; Wang, Shengrui 3; Niu, Yuan 3; Yang, Xi 2; Chen, Wenjie 1
刊名	JOURNAL OF LIMNOLOGY
	2015
卷号	74 期号:2 页码:310-323
关键词	Bacterial community composition organic matter denaturing gradient gel electrophoresis delta C-13 canonical correspondence analysis sediment
ISSN号	1129-5767
通讯作者	Xie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China.
英文摘要	Sediment cores taken from different areas of the mesotrophic Lake Erhai were analysed to investigate the vertical distribution of bacterial community composition (BCC), as well as physicochemical parameters. PCR-denaturing gradient gel electrophoresis (DGGE), stable carbon isotope (delta C-13), C/N atomic ratio and canonical correspondence analysis (CCA) were used to explore the relationships between the succession of bacterial communities and environmental variables, emphasising changes in the sources of organic matter (OM). The BCC in natural environments was characterised by DGGE of the 16S rRNA gene with subsequent sequencing of bands of interest. The CCA revealed that the depth-related variation in sediment bacterial communities in different areas of the lake was significantly influenced by varying environmental factors. The OM source, however, played an important role in structuring BCC at all sites. The DGGE banding patterns revealed that the abundance of Deltaproteobacteria decreased with accompanying elevated levels of C4 plant-derived organic carbon. The sequencing of DGGE bands suggested that the majority of the sequences were affiliated with common phylogenetic groups in lake sediments: Chloroflexi, Deltaproteobacteria and Firmicutes. Betaproteobacteria detected in our study appeared as a prominent phylotype in the upper sediment. The Shannon-Wiener diversity index of bacterial communities was directly affected by the OM source. Constant OM sources resulted in a stable higher diversity of bacterial communities and broader enzymatic capabilities to access OM. We conclude that the differences in the diversity of bacterial communities in sediments differing in their sources of OM were related to environmental variables (e.g. water level, river runoff and terrestrial vegetation composition). Our study provided insights into the relationships between natural BCC and OM sources, facilitating a better understanding of microbial community structure in lake sediment.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Limnology
研究领域[WOS]	Marine & Freshwater Biology
关键词[WOS]	GRADIENT GEL-ELECTROPHORESIS ; RIBOSOMAL-RNA FRAGMENTS ; CARBON ISOTOPES ; LATE QUATERNARY ; 16S RDNA ; BACTERIAL ; DIVERSITY ; BACTERIOPLANKTON ; DYNAMICS ; SHALLOW
收录类别	SCI
语种	英语
WOS记录号	WOS:000355151500011
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/23852]
专题	水生生物研究所_淡水生态学研究中心_期刊论文
作者单位	1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China 3.Chinese Res Inst Environm Sci, Res Ctr Lake Ecoenvironm, State Environm Protect Key Lab Lake Pollut Contro, Beijing 100012, Peoples R China
推荐引用方式 GB/T 7714	Xiong, Wei,Xie, Ping,Wang, Shengrui,et al. Sources of organic matter affect depth-related microbial community composition in sediments of Lake Erhai, Southwest China[J]. JOURNAL OF LIMNOLOGY,2015,74(2):310-323.
APA	Xiong, Wei,Xie, Ping,Wang, Shengrui,Niu, Yuan,Yang, Xi,&Chen, Wenjie.(2015).Sources of organic matter affect depth-related microbial community composition in sediments of Lake Erhai, Southwest China.JOURNAL OF LIMNOLOGY,74(2),310-323.
MLA	Xiong, Wei,et al."Sources of organic matter affect depth-related microbial community composition in sediments of Lake Erhai, Southwest China".JOURNAL OF LIMNOLOGY 74.2(2015):310-323.