Improved preparation and identification of aristolochic acid-DNA adducts by solid-phase extraction with liquid chromatography-tandem mass spectrometry

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Improved preparation and identification of aristolochic acid-DNA adducts by solid-phase extraction with liquid chromatography-tandem mass spectrometry
	Gao Haiyan ; Guo Fangqiu ; Feng Feng ; Yin Junfa ; Song Maoyong ; Wang Hailin
刊名	JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
	2009
卷号	21 期号:12 页码:1769-1776
关键词	aristolochic acid DNA adducts solid phase extraction quadrupole time-of-flight tandem mass spectrometry
中文摘要	Aristolochic acid (AA) is a known nephrotoxin and potential carcinogen, which can form covalent DNA adducts after metabolic activation in vivo and in vitro. A simple method for preparation and characterization of aristolochic acid-DNA adducts was developed. Four AA-adducts were synthesized by a direct reaction of AAI/AAII with 2'-deoxynucleosides. The reaction mixture was first cleaned-up and pre-concentrated using solid phase extraction (SPE), and further purified by a reversed-phase high performance liquid chromatography (HPLC). By the application of developed SPE procedure, matrices and byproducts in reaction mixture could be greatly reduced and adducts of high purity (more than 94% as indicated by HPLC) were obtained. The purified AA-DNA adducts were identified and characterized with liquid-electrospray ionization-quadrupole-time of flight-mass spectrometry (LC-ESI-Q-TOF-MS/MS) and LC-Diode array detector-fluorescence (LC-DAD-FL) analysis. This work provides a robust tool for possible large-scale preparation of AA-DNA adduct standards, which can promote the further studies on carcinogenic and mutagenic mechanism of aristolochic acids.
WOS记录号	WOS:000273080800022
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/21517]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Gao Haiyan,Guo Fangqiu,Feng Feng,et al. Improved preparation and identification of aristolochic acid-DNA adducts by solid-phase extraction with liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2009,21(12):1769-1776.
APA	Gao Haiyan,Guo Fangqiu,Feng Feng,Yin Junfa,Song Maoyong,&Wang Hailin.(2009).Improved preparation and identification of aristolochic acid-DNA adducts by solid-phase extraction with liquid chromatography-tandem mass spectrometry.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,21(12),1769-1776.
MLA	Gao Haiyan,et al."Improved preparation and identification of aristolochic acid-DNA adducts by solid-phase extraction with liquid chromatography-tandem mass spectrometry".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 21.12(2009):1769-1776.