Determination of Base Binding Strength and Base Stacking Interaction of DNA Duplex Using Atomic Force Microscope

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	Determination of Base Binding Strength and Base Stacking Interaction of DNA Duplex Using Atomic Force Microscope
	Zhang, Tianbiao; Zhang CL(张常麟); Dong ZL(董再励); Guan, Yifu
刊名	SCIENTIFIC REPORTS
	2015
卷号	5 页码:1-7
ISSN号	2045-2322
通讯作者	Guan, Yifu
产权排序	2
中文摘要	As one of the most crucial properties of DNA, the structural stability and the mechanical strength are attracting a great attention. Here, we take advantage of high force resolution and high special resolution of Atom Force Microscope and investigate the mechanical force of DNA duplexes. To evaluate the base pair hydrogen bond strength and base stacking force in DNA strands, we designed two modes (unzipping and stretching) for the measurement rupture forces. Employing k-means clustering algorithm, the ruptured force are clustered and the mean values are estimated. We assessed the influence of experimental parameters and performed the force evaluation for DNA duplexes of pure dG/dC and dA/dT base pairs. The base binding strength of single dG/dC and single dA/dT were estimated to be 20.0 +/- 0.2 pN and 14.0 +/- 0.3 pN, respectively, and the base stacking interaction was estimated to be 2.0 +/- 0.1 pN. Our results provide valuable information about the quantitative evaluation of the mechanical properties of the DNA duplexes.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	SINGLE-MOLECULE ; COMPLEMENTARY STRANDS ; OPTICAL TWEEZERS ; MAGNETIC BEADS ; DOUBLE HELIX ; STABILITY ; SPECTROSCOPY ; OLIGONUCLEOTIDE ; ELASTICITY ; RESOLUTION
收录类别	SCI
语种	英语
WOS记录号	WOS:000351181000007
内容类型	期刊论文
源URL	[http://ir.sia.ac.cn/handle/173321/16226]
专题	沈阳自动化研究所_机器人学研究室
推荐引用方式 GB/T 7714	Zhang, Tianbiao,Zhang CL,Dong ZL,et al. Determination of Base Binding Strength and Base Stacking Interaction of DNA Duplex Using Atomic Force Microscope[J]. SCIENTIFIC REPORTS,2015,5:1-7.
APA	Zhang, Tianbiao,Zhang CL,Dong ZL,&Guan, Yifu.(2015).Determination of Base Binding Strength and Base Stacking Interaction of DNA Duplex Using Atomic Force Microscope.SCIENTIFIC REPORTS,5,1-7.
MLA	Zhang, Tianbiao,et al."Determination of Base Binding Strength and Base Stacking Interaction of DNA Duplex Using Atomic Force Microscope".SCIENTIFIC REPORTS 5(2015):1-7.