Stearylamine liposome as a new efficient reagent for DNA transfection of eukaryotic cells | |
Wang, D; Jing, NH; Lin, QS | |
刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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1996 | |
卷号 | 226期号:2页码:450-455 |
英文摘要 | The efficiency of stearylamine (SA) liposome in transfecting DNA into eukarytic cells was evaluated and compared with that of the Lipofectin reagent and the calcium phosphate transfection method. Results with the 21 cell lines tested varied, depending on the cell type. The overall indication was that SA liposomes had certain advantages over the Lipofectin reagent and both of them were superior to the calcium phosphate method, showing the greatest effect in 8 different cell lines, while the calcium phosphate method was the best in only 4 cell lines. There were 2 cell lines which could only be transfected by SA liposomes, another 2 only by the calcium phosphate method, but none depended solely on the Lipofectin reagent. The number of cell lines failed to be transfected was 2 for SA liposomes, 3 for calcium phosphate method, and 4 for the Lipofectin reagent. The advantage of the SA liposome method is discussed. (C) 1996 Academic Press, Inc. |
学科主题 | Biochemistry & Molecular Biology; Biophysics |
类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
关键词[WOS] | GENES |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:A1996VJ52500026 |
内容类型 | 期刊论文 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/2746] ![]() |
专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
推荐引用方式 GB/T 7714 | Wang, D,Jing, NH,Lin, QS. Stearylamine liposome as a new efficient reagent for DNA transfection of eukaryotic cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,1996,226(2):450-455. |
APA | Wang, D,Jing, NH,&Lin, QS.(1996).Stearylamine liposome as a new efficient reagent for DNA transfection of eukaryotic cells.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,226(2),450-455. |
MLA | Wang, D,et al."Stearylamine liposome as a new efficient reagent for DNA transfection of eukaryotic cells".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 226.2(1996):450-455. |
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