Species-specific differences in the operational RNA code for aminoacylation of tRNA(Trp)

	Species-specific differences in the operational RNA code for aminoacylation of tRNA(Trp)
	Xu, F; Chen, XL; Xin, L; Chen, L; Jin, YX; Wang, DB
刊名	NUCLEIC ACIDS RESEARCH
	2001
卷号	29 期号:20 页码:4125-4133
通讯作者	Jin, YX (reprint author), Chinese Acad Sci, Shanghai Inst Life Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	Identity elements play essential roles in the recognition of tRNAs by their cognate aminoacyl-tRNA synthetase. An operational RNA code relates amino acids to specific sequences and structural features of tRNA acceptor stems. In this study, a series of tRNA(Trp) variants was prepared by in vitro transcription and their efficiencies of aminoacylation by tryptophan (k(cat)/K-m) were measured with the aid of Bacillus subtilis and human tryptophanyl-tRNA synthetases (TrpRS). The identity elements in the operational RNA code of human tRNA(Trp) were found to be: major element, discriminator base A73; minor elements, G1/C72 and U5/G68. From the cross-species aminoacylation assays, we conclude that the identity elements in tRNA(Trp) from B.subtilis and human all contribute to species-specific aminoacylation by TrpRS. Analyses of 22 TrpRS sequences covering three taxonomic domains (bacteria, eukarya and archaea) reveal that the sequences are divided into two evolutionarily distant groups. The same partition is also observed in the analyses of tRNA(Trp) acceptor stem sequences. Our data suggest that the two TrpRS groups may reflect co-adaptations needed to accommodate changes in the operational RNA code for tryptophan.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	IDENTITY ELEMENTS ; AMINO-ACIDS ; SYNTHETASE ; RECOGNITION ; NUCLEOTIDE ; ANTICODON ; SEQUENCES ; ALANINE ; MINIHELICES ; DIVERGENCE
收录类别	SCI
语种	英语
WOS记录号	WOS:000171796100004
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2713]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xu, F,Chen, XL,Xin, L,et al. Species-specific differences in the operational RNA code for aminoacylation of tRNA(Trp)[J]. NUCLEIC ACIDS RESEARCH,2001,29(20):4125-4133.
APA	Xu, F,Chen, XL,Xin, L,Chen, L,Jin, YX,&Wang, DB.(2001).Species-specific differences in the operational RNA code for aminoacylation of tRNA(Trp).NUCLEIC ACIDS RESEARCH,29(20),4125-4133.
MLA	Xu, F,et al."Species-specific differences in the operational RNA code for aminoacylation of tRNA(Trp)".NUCLEIC ACIDS RESEARCH 29.20(2001):4125-4133.