The relationship between the connecting peptide of recombined single chain insulin and its biological function

	The relationship between the connecting peptide of recombined single chain insulin and its biological function
	Huang, YD; Liang, ZH; Feng, YM
刊名	SCIENCE IN CHINA SERIES C-LIFE SCIENCES
	2001
卷号	44 期号:6 页码:593-600
关键词	insulin single chain insulin receptor binding growth promoting GB2H6 cell line
通讯作者	Feng, YM (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,
英文摘要	To investigate the relationship between the biological activity of recombined single chain insulin and the length of the connecting peptide, we designed and prepared three single chain insulin molecules, namely, PIP, [A](5)PIP and [A](10)PIP, by site-directed mutagenesis, in which B30 and A1 were linked through dipeptide A-K, heptapeptide A-A-A-A-A-A-K, and dodecapeptide A-A-A-A-A-A-A-A-A-A-A-K, respectively. Their receptor binding capacities were 0.14%, 14.3% and 11.1% of that of insulin respectively and their in vivo biological activities were in consistence with their receptor binding capacity; whereas their growth promoting activities were 17%, 116.3% and 38% of that of insulin. These results suggested the following conclusions. (i) The recombined single chain insulin could also possess the same metabolic and mitogenic function as insulin. (ii) The receptor binding capacity of recombined single chain insulin to insulin receptor was closely related to the length and amino acid composition of the connecting peptide and could change from 0 to 100% of insulin depending on the different connecting peptides. This result further illustrated the necessity of B chain C-terminus swaying away from A chain N-terminus when insulin binds to its receptor. (iii) The mitogenic activity of recombined single chain insulin also depended on the length and the amino acid composition of the connecting peptide and was higher than its metabolic activity.
学科主题	Life Sciences & Biomedicine - Other Topics
类目[WOS]	Biology
关键词[WOS]	GROWTH-FACTOR-I ; RECEPTOR-BINDING ; SIDE-CHAIN ; PROTEIN ; CONFORMATION ; INVITRO ; HYBRID ; INVIVO
收录类别	SCI
语种	英语
WOS记录号	WOS:000175029600005
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2679]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Huang, YD,Liang, ZH,Feng, YM. The relationship between the connecting peptide of recombined single chain insulin and its biological function[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2001,44(6):593-600.
APA	Huang, YD,Liang, ZH,&Feng, YM.(2001).The relationship between the connecting peptide of recombined single chain insulin and its biological function.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,44(6),593-600.
MLA	Huang, YD,et al."The relationship between the connecting peptide of recombined single chain insulin and its biological function".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 44.6(2001):593-600.