Functional analysis of helicase gene promoter and homologous region 3 enhancer in Bombyx mori nuclear polyhedrosis virus

	Functional analysis of helicase gene promoter and homologous region 3 enhancer in Bombyx mori nuclear polyhedrosis virus
	Xiao, QL; Zhang, ZF; Yi, YZ; He, JL; Wu, XF
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2001
卷号	33 期号:5 页码:525-530
关键词	Bombyx mori nuclear polyhedrosis virus helicase gene promoter enhancer transient expression
通讯作者	,
英文摘要	The promoter of the helicase gene, including 510 bp upstream of ATG, was cloned and sequenced, and was found that it had both early and late RNA initiation sites. The initiation codon ATG was deleted by using point mutation. Luciferase gene, as a reporter gene, was fused with the promoter region to construct the plsmid pBmhel510luc. When pBmhel510luc was transfected into Bm-5 and Sf-21 cell lines, the helicase gene promoter was recognized by cellular RNA polymerase and transactivated by viral factors. Baculovirus homologous regions (hrs) act as viral DNA replication start sites, which also have been shown to alter the rate of transcription for cis-linked promoters. BmNPV hr3 was cloned into a downstream site of luc gene, to study the effect of this enhancer on hel510 promoter activity. The transient expression in transfected insect cell lines and silkworm larvae indicated that hr3 could enhance the transcriptional level of hel510 promoter by about 7 000 and 1 000 fold, respectively.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	NUCLEOTIDE-SEQUENCE ; P143 GENE ; DNA-SEQUENCE ; EXPRESSION ; TRANSCRIPTION ; PROTEIN
收录类别	SCI
语种	中文
WOS记录号	WOS:000171403600009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2669]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xiao, QL,Zhang, ZF,Yi, YZ,et al. Functional analysis of helicase gene promoter and homologous region 3 enhancer in Bombyx mori nuclear polyhedrosis virus[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(5):525-530.
APA	Xiao, QL,Zhang, ZF,Yi, YZ,He, JL,&Wu, XF.(2001).Functional analysis of helicase gene promoter and homologous region 3 enhancer in Bombyx mori nuclear polyhedrosis virus.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(5),525-530.
MLA	Xiao, QL,et al."Functional analysis of helicase gene promoter and homologous region 3 enhancer in Bombyx mori nuclear polyhedrosis virus".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.5(2001):525-530.