Overexpression of a novel gene, Cms1, can rescue the growth arrest of a Saccharomyces cerevisiae mcm10 suppressor

	Overexpression of a novel gene, Cms1, can rescue the growth arrest of a Saccharomyces cerevisiae mcm10 suppressor
	Wang, JW; Wu, JR
刊名	CELL RESEARCH
	2001
卷号	11 期号:4 页码:285-291
关键词	Saccharomyces cerevisiae DNA replication Mcm10 Cms1 cell cycle
通讯作者	Wu, JR (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Res Ctr Life Sci,Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	MCM10 protein is an essential replication factor involved in the initiation of DNA replication. A mcm10 mutant (mcm10-1) of budding yeast shows a growth arrest at 37 degreesC, In the present work, we have isolated a mcm10-1 suppressor strain, which grows at 37 degreesC. Interestingly, this mcm10-1 suppressor undergoes cell cycle arrest at 14 degreesC. A novel gene, YLR003c, is identified by high-copy complementation of this suppressor. We called it as Cms1 (Complementation of Mcm 10 Suppressor). Furthermore, the experiments of transformation show that cells of mcm10-1 suppressor with high-copy plasmid but not low-copy plasmid grow at 14 degreesC, indicating that overexpression of Cms1 can rescue the growth arrest of this mcm10 suppressor at non-permissive temperature. These results suggest that CMS1 protein may functionally interact with MCM10 protein and play a role in the regulation of DNA replication and cell cycle control.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	DNA HELICASE ACTIVITY ; REPLICATION ; PROTEINS ; YEAST ; TRANSFORMATION ; INITIATION ; ORIGINS
收录类别	SCI
语种	英语
WOS记录号	WOS:000173058800006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2639]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, JW,Wu, JR. Overexpression of a novel gene, Cms1, can rescue the growth arrest of a Saccharomyces cerevisiae mcm10 suppressor[J]. CELL RESEARCH,2001,11(4):285-291.
APA	Wang, JW,&Wu, JR.(2001).Overexpression of a novel gene, Cms1, can rescue the growth arrest of a Saccharomyces cerevisiae mcm10 suppressor.CELL RESEARCH,11(4),285-291.
MLA	Wang, JW,et al."Overexpression of a novel gene, Cms1, can rescue the growth arrest of a Saccharomyces cerevisiae mcm10 suppressor".CELL RESEARCH 11.4(2001):285-291.