Investigating the function of Akt by tet-off inducible expression system

	Investigating the function of Akt by tet-off inducible expression system
	Zhai, QW; Ji, HB; Zheng, ZC; Sun, LY; Liu, XY
刊名	CHINESE SCIENCE BULLETIN
	2001
卷号	46 期号:3 页码:222-225
关键词	inducible expression system Akt apoptosis
通讯作者	Zheng, ZC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,
英文摘要	A tet-off inducible cell line named BBT derived from BA/F3 beta cell line was constructed and the effect of this inducible expression system was significant when detected by tet-off responded luciferase reporter gene assay. Then tet-off responded Akt expression plasmid was transfected into BET cells, and the stable cell lines were screened. The result of Northern blot showed that the expression of akt was significantly inducible. The clone with the best inducible effect was selected and named BBA for investigating the function of Akt. We found that Akt could significantly inhibit zinc-induced decrease of cell viability when assayed by MTT method. And the flow cytometric analysis showed that Akt could markedly repress zinc-induced apoptosis.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	GENE-EXPRESSION ; PHOSPHOINOSITIDE 3-KINASE ; MAMMALIAN-CELLS ; APOPTOSIS ; PHOSPHORYLATION ; REPRESSOR ; SURVIVAL ; PATHWAY
收录类别	SCI
语种	英语
WOS记录号	WOS:000167179800014
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2635]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhai, QW,Ji, HB,Zheng, ZC,et al. Investigating the function of Akt by tet-off inducible expression system[J]. CHINESE SCIENCE BULLETIN,2001,46(3):222-225.
APA	Zhai, QW,Ji, HB,Zheng, ZC,Sun, LY,&Liu, XY.(2001).Investigating the function of Akt by tet-off inducible expression system.CHINESE SCIENCE BULLETIN,46(3),222-225.
MLA	Zhai, QW,et al."Investigating the function of Akt by tet-off inducible expression system".CHINESE SCIENCE BULLETIN 46.3(2001):222-225.