Differential expression of CCAAT/enhancer-binding protein-delta (C/EBP delta) in rat androgen-dependent tissues and human prostate cancer

	Differential expression of CCAAT/enhancer-binding protein-delta (C/EBP delta) in rat androgen-dependent tissues and human prostate cancer
	Yang, GY; Gregory, CW; Shang, Q; O'Brien, DA; Zhang, YL
刊名	JOURNAL OF ANDROLOGY
	2001
卷号	22 期号:3 页码:471-480
关键词	transcription factor testosterone
通讯作者	Zhang, YL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	CCAAT/enhancer-binding protein delta (C/EBP delta) is a nuclear transcription factor that regulates cellular growth and differentiation. In this study we demonstrate that C/EBP delta gene expression is differentially regulated in rat androgen-dependent tissues and human prostate cancer. C/EBP delta messenger RNA (mRNA) levels were very low in adult rat ventral prostate, epididymis, and testis. In ventral prostate and epididymis, expression of C/EBP delta mRNA increased more than sixfold when testicular testosterone was eliminated by surgical castration or treatment with ethane-1, 2-dimethanesulfonate (EDS). Testosterone replacement reduced C/EBP delta mRNA levels to near control values in both tissues. CWR22 is a human prostate cancer xenograft that mimics biological characteristics of androgen-dependent and androgen-independent human prostate cancer. In androgen-dependent CWR22 tumors, expression of C/EBP delta mRNA declined in response to castration. Both C/EBP delta mRNA and protein levels increased following testosterone administration. However, C/ EBP delta mRNA and protein levels were variable in recurrent CWR22 tumors growing in the absence of testicular androgen for similar to5 months. C/EBP delta expression was also variable in androgen-independent human prostate carcinomas (n = 3), although mRNA levels were substantially lower than those in androgen-dependent tumors (n = 3). These studies demonstrate that androgen down-regulates C/EBP delta levels in androgen-dependent rat tissues, but induces C/EBP delta expression in androgen-dependent human prostate cancer. Deregulation of C/EBP delta occurs when prostate cancer progresses to the androgen-independent state.
学科主题	Endocrinology & Metabolism
类目[WOS]	Andrology
关键词[WOS]	LEUCINE-ZIPPER PROTEINS ; GROWTH-FACTOR-ALPHA ; VENTRAL PROSTATE ; TRANSCRIPTIONAL REGULATION ; ADIPOCYTE DIFFERENTIATION ; NUCLEAR-PROTEIN ; TRANSFORMING GROWTH-FACTOR-BETA-1 ; GENE-TRANSCRIPTION ; CELL-CYCLE ; RECEPTOR
收录类别	SCI
语种	英语
WOS记录号	WOS:000168203300016
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2631]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yang, GY,Gregory, CW,Shang, Q,et al. Differential expression of CCAAT/enhancer-binding protein-delta (C/EBP delta) in rat androgen-dependent tissues and human prostate cancer[J]. JOURNAL OF ANDROLOGY,2001,22(3):471-480.
APA	Yang, GY,Gregory, CW,Shang, Q,O'Brien, DA,&Zhang, YL.(2001).Differential expression of CCAAT/enhancer-binding protein-delta (C/EBP delta) in rat androgen-dependent tissues and human prostate cancer.JOURNAL OF ANDROLOGY,22(3),471-480.
MLA	Yang, GY,et al."Differential expression of CCAAT/enhancer-binding protein-delta (C/EBP delta) in rat androgen-dependent tissues and human prostate cancer".JOURNAL OF ANDROLOGY 22.3(2001):471-480.