Expression of human angiostatin in Pichia pastoris and the detection of its anti-angiogenic activity

	Expression of human angiostatin in Pichia pastoris and the detection of its anti-angiogenic activity
	Xin, L; Zhang, L; Xu, R; Zhang, Q; Ye, Q; Li, ZP; Gan, RB
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2001
卷号	33 期号:3 页码:291-295
关键词	angiostatin Pichia pastoris multiple copy integration inhibit endothelial cell
通讯作者	,
英文摘要	Human angiostatin cDNA was amplified from human hepatoma cell line HepG2 using RT-PCR and was cloned into pPIC9K vector. Recombinant Pichia pastoris strain with 4 copies of angiostatin gene was obtained. Recombinant protein was purified by lysine-affinity Sepharose column and the finally purified angiostatin was 25 mg/L, higher than previously reported 17 mg/L. Amino acid sequence analysis revealed the identity of our protein the same with that previously reported. Recombinant angiostatin inhibited specifically the proliferation of bovine aortic endothelial cell stimulated by bFGF, with ED50 being about 3 mg/L.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	ENDOTHELIAL-CELLS ; PLASMINOGEN ; INHIBITOR ; SUPPRESSION ; CARCINOMA
收录类别	SCI
语种	中文
WOS记录号	WOS:000168839400007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2629]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xin, L,Zhang, L,Xu, R,et al. Expression of human angiostatin in Pichia pastoris and the detection of its anti-angiogenic activity[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(3):291-295.
APA	Xin, L.,Zhang, L.,Xu, R.,Zhang, Q.,Ye, Q.,...&Gan, RB.(2001).Expression of human angiostatin in Pichia pastoris and the detection of its anti-angiogenic activity.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(3),291-295.
MLA	Xin, L,et al."Expression of human angiostatin in Pichia pastoris and the detection of its anti-angiogenic activity".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.3(2001):291-295.