Inhibition of protein tyrosine phosphatases suppresses P-selectin exocytosis in activated human platelets

	Inhibition of protein tyrosine phosphatases suppresses P-selectin exocytosis in activated human platelets
	Chen, M; Geng, JG
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2001
卷号	286 期号:4 页码:831-838
关键词	phenylarsine oxide P-selectin platelet exocytosis
通讯作者	Geng, JG (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Room 505,320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	P-selectin (CD62P), a cell adhesion molecule for most leukocytes, is stored in the a-granules of platelets and the Weibel-Palade bodies of endothelial cells. Upon thrombogenic and inflammatory challenges, P-selectin is rapidly expressed, by exocytosis, on activated platelets and stimulated endothelial cells. However, little is known for the molecular mechanisms governing the regulation of the rapid mobilization of P-selectin in these cells. Here we show that phenylarsine oxide (PAO) and diamide (both were inhibitors for protein tyrosine phosphatases), but not genistein (an inhibitor for protein tyrosine kinases), adenosine, wortmannin and LY294002 (all were inhibitors for phosphatidylinositol 3- and 4-kinases), could inhibit P-selectin exocytosis on activated platelets and could abolish the P-selectin mediated aggregation of activated platelets to neutrophils. However, PAO did not attenuate the P-selectin mediated adhesion of human promyeloid HL-60 cells on the stimulated endothelial cells under flow conditions. Further, PAO had no detectable effects on the exocytosis of P-selectin in the stimulated endothelial cells. These results indicate that protein tyrosine phosphatases are necessary for P-selectin exocytosis on the activated platelets, but not on the stimulated endothelial cells, and suggest that inhibitors for protein tyrosine phosphatases may be potentially valuable for treatment of platelet/leukocyte aggregation. (C) 2001 Academic Press.
学科主题	Biochemistry & Molecular Biology ; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	GRANULE MEMBRANE-PROTEIN ; HUMAN-ENDOTHELIAL-CELLS ; WEIBEL-PALADE BODIES ; PHOSPHATIDYLINOSITOL 4-KINASE ; MONOCLONAL-ANTIBODY ; NEUTROPHIL ADHESION ; SECRETION ; GMP-140 ; PHOSPHORYLATION ; EXPRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000170911700027
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2620]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Chen, M,Geng, JG. Inhibition of protein tyrosine phosphatases suppresses P-selectin exocytosis in activated human platelets[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2001,286(4):831-838.
APA	Chen, M,&Geng, JG.(2001).Inhibition of protein tyrosine phosphatases suppresses P-selectin exocytosis in activated human platelets.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,286(4),831-838.
MLA	Chen, M,et al."Inhibition of protein tyrosine phosphatases suppresses P-selectin exocytosis in activated human platelets".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 286.4(2001):831-838.