Interactions among subunits of human Arp2/3 complex: p20-Arc as the hub

	Interactions among subunits of human Arp2/3 complex: p20-Arc as the hub
	Zhao, XS; Yang, ZY; Qian, M; Zhu, XL
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2001
卷号	280 期号:2 页码:513-517
关键词	actin subunit Arp2/3 complex yeast two-hybrid assay
通讯作者	Zhu, XL (reprint author), Chinese Acad Sci, Shanghai Res Ctr Life Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	The Arp2/3 complex is critical for nucleation and crosslinking of actin filaments, To gain insight into its subunit topology and assembly pathway, we systematically examined interactions among subunits of human Arp2/3 complex by yeast two-hybrid assays. It was shown that P20-Arc was able to interact with p21-Arc, p34-Arc, and p16-Arc, respectively. In contrast, p41-Arc only interacted with p20-Arc/p16-Arc heterodimer. In addition, we found that structural integrity was important for association between p20-Arc and p21-Arc, while the N-terminal half of p34-Arc was dispensable for its binding to p20-Arc. Our data suggest a key role of p20-Arc and a multistep pathway for the complex formation. (C) Academic Press.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	ACTIN ; PROTEIN ; ACANTHAMOEBA ; NUCLEATION ; FILAMENTS ; REGIONS ; WASP
收录类别	SCI
语种	英语
WOS记录号	WOS:000166697100015
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2614]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhao, XS,Yang, ZY,Qian, M,et al. Interactions among subunits of human Arp2/3 complex: p20-Arc as the hub[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2001,280(2):513-517.
APA	Zhao, XS,Yang, ZY,Qian, M,&Zhu, XL.(2001).Interactions among subunits of human Arp2/3 complex: p20-Arc as the hub.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,280(2),513-517.
MLA	Zhao, XS,et al."Interactions among subunits of human Arp2/3 complex: p20-Arc as the hub".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 280.2(2001):513-517.