Substrate-structure dependence of ribotoxins on cleaving RNA in C-camphora ribosome

	Substrate-structure dependence of ribotoxins on cleaving RNA in C-camphora ribosome
	Zhang, AH; Tang, S; Liu, WY
刊名	JOURNAL OF NATURAL TOXINS
	2001
卷号	10 期号:2 页码:119-125
通讯作者	Liu, WY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	Cinnamomin, a type II ribosome-inactivating protein (RIP) isolated from the seeds of Camphora tree (Cinnamomum camphora), could not inactivate its own tautologous) ribosome. Among five RIPs (Cinnamomin A-chain, ricin A-chain, trichosanthin, gelonin, and soporin-S6) tested, only saporin-S6 could cleave the N-glycosidic bond of RNA in C. camphora ribosome to release a specific RNA fragment (R-fragment) after treatment with aniline, which was shorter than that from rat fiver ribosome. The amount of saporin-S6 to inactivate C. camphora ribosome was about 1000 times higher than that required for rat liver ribosome. Extra-ribosomal factors (S-100) in the post-ribosomal supernatant could not promote RNA N-glycosidase activity of cinnamomin and gelonin to C. camphora ribosome. These results indicated that there were some changes in the microenvironments of Sarcin/Ricin domain of C. camphora ribosome that abolished the recognition and catalysis of many RIPs. In addition, the length of C. camphora 5.8S ribosomal RNA was found to be longer than that of rat 5.8S ribosomal RNA.
学科主题	Biochemistry & Molecular Biology; Toxicology
类目[WOS]	Biochemistry & Molecular Biology ; Toxicology
关键词[WOS]	INACTIVATING PROTEINS ; EUKARYOTIC RIBOSOMES ; N-GLYCOSIDASES ; TRNA(TRP) ; MECHANISM ; GELONIN ; SEEDS
收录类别	SCI
语种	英语
WOS记录号	WOS:000169402600005
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2603]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, AH,Tang, S,Liu, WY. Substrate-structure dependence of ribotoxins on cleaving RNA in C-camphora ribosome[J]. JOURNAL OF NATURAL TOXINS,2001,10(2):119-125.
APA	Zhang, AH,Tang, S,&Liu, WY.(2001).Substrate-structure dependence of ribotoxins on cleaving RNA in C-camphora ribosome.JOURNAL OF NATURAL TOXINS,10(2),119-125.
MLA	Zhang, AH,et al."Substrate-structure dependence of ribotoxins on cleaving RNA in C-camphora ribosome".JOURNAL OF NATURAL TOXINS 10.2(2001):119-125.