Bradykinin induces protein kinase C-dependent activation of phospholipase D in A-431 cells

	Bradykinin induces protein kinase C-dependent activation of phospholipase D in A-431 cells
	Chen, JS; Song, JG
刊名	IUBMB LIFE
	2001
卷号	51 期号:1 页码:49-56
关键词	bradykinin diglyceride EGF phospholipase D PMA protein kinase C
通讯作者	Song, JG (reprint author), Chinese Acad Sci, Shanghai Inst Biochem, Box 25,320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	The protein kinase C involvement in bradykinin (BK)-induced phospholipase D (PLD) activation in A-431 cells was examined. Treatment of cells with BK induced the rapid activation of intracellular PLD activity, The PLD activation induced by BK was blocked by pretreatment of A-431 cells with staurosporine, or by prolonged treatment with phorbol-12-myristate-13-acetate (PMA), PKC inhibitors Re-31-8220 and bisindolylmaleimide 1, showed the same inhibitory effects on the BK-stimulated increase of PLD activity, indicating a role of PKC in this activation process, Similar results were observed in PMA-induced PLD activation, In contrast, PKC down-regulation or PKC inhibitors had no obvious effect on the PLD activation stimulated by epidermal growth factor (EGF), Furthermore, rottlerin and Go 6976, the PKC inhibitors specific for PKC-delta, -alpha and -betaI, respectively, markedly inhibited the PLD activity stimulated by BK, These results indicated that PKC, at least PKC-delta and Ca2+-dependent PKC-alpha or -betaI, plays an important role in BK-induced but not EGF-induced PLD activation in A-431 cells.
学科主题	Biochemistry & Molecular Biology ; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	PHOSPHORYLATION ; RECEPTOR ; ALPHA ; PHOSPHATIDYLCHOLINE ; FIBROBLASTS ; STIMULATION ; MECHANISMS ; HYDROLYSIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000169228900008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2591]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Chen, JS,Song, JG. Bradykinin induces protein kinase C-dependent activation of phospholipase D in A-431 cells[J]. IUBMB LIFE,2001,51(1):49-56.
APA	Chen, JS,&Song, JG.(2001).Bradykinin induces protein kinase C-dependent activation of phospholipase D in A-431 cells.IUBMB LIFE,51(1),49-56.
MLA	Chen, JS,et al."Bradykinin induces protein kinase C-dependent activation of phospholipase D in A-431 cells".IUBMB LIFE 51.1(2001):49-56.