Enhancement of macrophage cytotoxicity by overexpression of exogenous NF-IL6 gene

	Enhancement of macrophage cytotoxicity by overexpression of exogenous NF-IL6 gene
	Liu, HD; Liu, DG
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2001
卷号	33 期号:1 页码:35-40
关键词	murine peritoneal macrophage NF-IL6 tumor cytotoxicity DEAE-dextran mediated transfection alkaline phosphatase assay
通讯作者	,
英文摘要	NF-IL6 (Nuclear factor for IL-6 expression) is involved in inflammatory reaction, expression of acute-phase proteins and cytokines, apoptosis and suppression of tumor cells, and maintenance of macrophage immunological functions. To investigate the role of highly expressed exogenous NF-IL6 in macrophage tumor cytotoxicity, a recombinant expression plasmid, pCN, which harbored the coding region of NF-IL6, was transfected into murine primary cultured peritoneal resident macrophages by an improved DEAE-dextran method. Western blot showed the high expression of NF-IL6 in these macrophages. Then the tumor cytotoxicity of the NF-IL6-overexpressing macrophages from normal and nude mice was measured by an alkaline phosphatase assay, using the human hepatocarcinoma cell line SMMC 7721 as target cells. Results showed that the overexpression of NF-IL6 enhanced the tumor cytotoxicity in both types of macrophages, demonstrating that the expression level of the NF-IL6 gene was directly related to the tumoricidal activity in these cells.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	MEDIATED TRANSFECTION ; NUCLEAR FACTOR ; IFN-GAMMA ; EXPRESSION ; LIPOPOLYSACCHARIDE ; CELLS
收录类别	SCI
语种	中文
WOS记录号	WOS:000166598200007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2583]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liu, HD,Liu, DG. Enhancement of macrophage cytotoxicity by overexpression of exogenous NF-IL6 gene[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(1):35-40.
APA	Liu, HD,&Liu, DG.(2001).Enhancement of macrophage cytotoxicity by overexpression of exogenous NF-IL6 gene.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(1),35-40.
MLA	Liu, HD,et al."Enhancement of macrophage cytotoxicity by overexpression of exogenous NF-IL6 gene".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.1(2001):35-40.