In vitro triplex formation and functional analysis of TFOs designed against human c-sis/PDGF-B proto-oncogene

	In vitro triplex formation and functional analysis of TFOs designed against human c-sis/PDGF-B proto-oncogene
	Liu, J; Xu, RH; Jin, YX; Wang, DB
刊名	SCIENCE IN CHINA SERIES C-LIFE SCIENCES
	2001
卷号	44 期号:1 页码:83-91
关键词	c-sis/PDGF-B proto-oncogene tripler-forming oligonucleotides (TFO)
通讯作者	Jin, YX (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,
英文摘要	PDGF (platelet derived growth factor) has been shown to play an important role in tumorigenesis, tumor growth, atherosclerosis and inflammation and other various pathologic settings. PDGF-B chain gene is 92% homologous to v-sis oncogene of the simian sarcoma virus. Thus PDGF-B gene is also called c-sis proto-oncogene. This report provides 3 TFOs (triplex-forming oligonucleotides) to inhibit the expression of c-sis/PDGF-B gene. The results from gel mobility shift analysis, in vitro transcription, DNase I footprinting and protein binding assays demonstrate that the TFOs we designed can form sequence-specific stable tripler with the target, and can effectively suppress the downstream gene transcription and inhibit transcription factors binding. They can be used for preparation of drugs to inhibit tumor growth and for the therapy of atherosclerosis, inflammation, etc.
学科主题	Life Sciences & Biomedicine - Other Topics
类目[WOS]	Biology
关键词[WOS]	OSTEOSARCOMA CELLS ; GENE ; TRANSCRIPTION
收录类别	SCI
语种	英语
WOS记录号	WOS:000167665600011
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2581]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liu, J,Xu, RH,Jin, YX,et al. In vitro triplex formation and functional analysis of TFOs designed against human c-sis/PDGF-B proto-oncogene[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2001,44(1):83-91.
APA	Liu, J,Xu, RH,Jin, YX,&Wang, DB.(2001).In vitro triplex formation and functional analysis of TFOs designed against human c-sis/PDGF-B proto-oncogene.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,44(1),83-91.
MLA	Liu, J,et al."In vitro triplex formation and functional analysis of TFOs designed against human c-sis/PDGF-B proto-oncogene".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 44.1(2001):83-91.