NC1 domain of human type VIII collagen (alpha 1) inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis

	NC1 domain of human type VIII collagen (alpha 1) inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis
	Xu, R; Yao, ZY; Xin, L; Zhang, Q; Li, TP; Gan, RB
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2001
卷号	289 期号:1 页码:264-268
关键词	vastatin type VIII collagen NC1 domain angiogenesis inhibitor apoptosis
通讯作者	Gan, RB (reprint author), Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Inst Biochem & Cell Biol, 320 Yue Yang Rd,POB 55, Shanghai 200031, Peoples R China.,
英文摘要	Endostatin, a natural angiogenesis inhibitor, had been identified for years. It opened a new approach for cancer therapy. Sequence analysis reveled that endostatin is the NC1 domain (non-triple-helical domain) of collagen XVIII. In this report, the cDNA of NC1 domain of type VIII collagen (alpha 1) was cloned and expressed as soluble form in Escherichia coli. The recombinant protein was purified with Ni-NTA agarose column and named as vastatin. It inhibited the proliferation of bovine aortic endothelial (BAE) cell stimulated by basic fibroblast growth factor (bFGF) in a dose-dependent manner. The ED50 of vastatin was 0.6 mug/ml, while the ED50 of endostatin was 0.5 mug/ml. Treatment of BAE cell with vastatin caused G(0)-G(1) arrest and cell apoptosis. It is interesting that sequence analysis showed that there was only about 12% amino acid sequence homology between vastatin and endostatin. The structure-function relationship of these angiogenesis molecules remains to be elucidated. (C) 2001 Academic Press.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	TUMOR-GROWTH ; ENDOSTATIN ; ANGIOGENESIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000172451100044
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2572]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xu, R,Yao, ZY,Xin, L,et al. NC1 domain of human type VIII collagen (alpha 1) inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2001,289(1):264-268.
APA	Xu, R,Yao, ZY,Xin, L,Zhang, Q,Li, TP,&Gan, RB.(2001).NC1 domain of human type VIII collagen (alpha 1) inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,289(1),264-268.
MLA	Xu, R,et al."NC1 domain of human type VIII collagen (alpha 1) inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 289.1(2001):264-268.