Cloning and characterization of a mouse liver-specific gene mfrep-1, upregulated in liver regeneration

	Cloning and characterization of a mouse liver-specific gene mfrep-1, upregulated in liver regeneration
	Yan, J; Ying, H; Gu, F; He, J; Li, YL; Liu, HM; Xu, YH
刊名	CELL RESEARCH
	2002
卷号	12 期号:1 页码:353-361
关键词	mfrep-1 in silico cloning liver regeneration liver-specific expression
通讯作者	Xu, YH (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Lab Mol & Cellular Oncol, Shanghai Inst Biol Sci, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,yhxu@sunm.shcnc.ac.cn
英文摘要	Human fibrinogen-related protein-1/liver fibrinogen-related protein-1 (HFREP-1/LFIRE-1), a liver-specific protein, is a member of fibrinogen superfamily that exerts various biological activities. However, the function of HFREP-1/LFIRE-1 in liver remains unknown. Here we isolated its mouse ortholog gene-mouse fibrinogen-related protein-1 (mfrep-1), which encoded 314 amino acids, exhibiting 80.4% similarity to HFREP-1/LFIRE-1. Northern blot analysis revealed that 1.2-kb mfrep-1 mRNA was detected selectively in mouse liver. To explore the function of MFREP-1, we examined the levels of mfrep-1 mRNA during regeneration after 70% partial hepatectomy (PHx) in mice. mfrep-1 mRNA increased in the regenerating liver and reached the first shoulder peak at 2-4 h after PHx. Cycloheximide pretreatment could suppress the induction of mfrep-1, indicating the up-regulation of this gene need de novo protein synthesis. Its mRNA continued to elevate at 6 h thereafter and reached the second peak at 24 h. The enhanced expression of mfrep-1 maintained high until 72 h and then declined slowly to the basal level. Immunohistochemistry assessment confirmed the up-regulated expression of MFREP-1 protein in parenchymal cells during liver regeneration. These data suggested that MFREP-1 might play an important role in liver regeneration and be involved in the regulation of cell growth.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	PARTIAL-HEPATECTOMY ; EXPRESSION ; GROWTH ; RAT ; IDENTIFICATION ; INJURY ; MICE
收录类别	SCI
语种	英语
WOS记录号	WOS:000180476800005
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2562]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yan, J,Ying, H,Gu, F,et al. Cloning and characterization of a mouse liver-specific gene mfrep-1, upregulated in liver regeneration[J]. CELL RESEARCH,2002,12(1):353-361.
APA	Yan, J.,Ying, H.,Gu, F.,He, J.,Li, YL.,...&Xu, YH.(2002).Cloning and characterization of a mouse liver-specific gene mfrep-1, upregulated in liver regeneration.CELL RESEARCH,12(1),353-361.
MLA	Yan, J,et al."Cloning and characterization of a mouse liver-specific gene mfrep-1, upregulated in liver regeneration".CELL RESEARCH 12.1(2002):353-361.