Identification of up-regulated genes in human uterine leiomyoma by suppression subtractive hybridization

	Identification of up-regulated genes in human uterine leiomyoma by suppression subtractive hybridization
	Li, B; Zhang, YL
刊名	CELL RESEARCH
	2002
卷号	12 期号:1 页码:215-221
关键词	human uterine leiomyoma suppression subtractive hybridization up-regulated gene in uterine leiomyoma screening library.
通讯作者	Zhang, YL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,
英文摘要	In searching for differentially expressed genes in human uterine leiomyomas (ULs), suppression subtractive hybridization was used to construct an UL up-regulated library, which turned out to represent 88 genes. After two rounds of screening by reverse Northern analysis, twenty genes were proved to be up-regulated, including seventeen known genes and three genes with unknown function. All these genes were firstly associated with UL. Three genes with notable difference were selected for Northern confirmation. Our results proved the authenticity of the twenty genes. One gene named Phospholipase A2 (PLA(2)) showed up-regulation in 4/6 of the patients and investigation of tissue distribution indicated that it had obvious expression in prostate, testis, liver, heart and skeletal muscle.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	REARRANGEMENTS ; ABNORMALITIES ; CYTOGENETICS ; EXPRESSION ; PROGESTERONE ; ESTROGEN
收录类别	SCI
语种	英语
WOS记录号	WOS:000178031600005
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2557]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, B,Zhang, YL. Identification of up-regulated genes in human uterine leiomyoma by suppression subtractive hybridization[J]. CELL RESEARCH,2002,12(1):215-221.
APA	Li, B,&Zhang, YL.(2002).Identification of up-regulated genes in human uterine leiomyoma by suppression subtractive hybridization.CELL RESEARCH,12(1),215-221.
MLA	Li, B,et al."Identification of up-regulated genes in human uterine leiomyoma by suppression subtractive hybridization".CELL RESEARCH 12.1(2002):215-221.