Development of the diagnostic immunoassay to detect anti-PreS1(21-47aa) antibody - a marker suggesting the health improvement of hepatitis B patients

	Development of the diagnostic immunoassay to detect anti-PreS1(21-47aa) antibody - a marker suggesting the health improvement of hepatitis B patients
	Wei, J; Liu, XJ; Wang, YQ; Lu, ZM; Li, GD; Wang, Y; Zhang, ZC
刊名	CLINICA CHIMICA ACTA
	2002
卷号	317 期号:1 页码:159-169
关键词	hepatitis B virus PreS1 antibody diagnostic marker immunoassay ELISA
通讯作者	Zhang, ZC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,
英文摘要	Background: A new immunoassay has been developed for the detection of the anti-PreS1(21-47aa) antibody in sera of hepatitis B virus (HBV)-infected patients. Anti-PreS1(21-47aa) antibody involves virus neutralization and is a new marker for diagnosing acute and chronic B hepatitis. Methods: The expression plasmids pGEXS I and pGEXS II, which expressed glutathione S-transferase (GST) fusion proteins containing a copy of PreS1(21-47aa) peptide and two orderly joined copies of PreS1(21-47aa) peptide, were constructed. The soluble expression products were purified by affinity chromatography. Results: The two PreS1(21-47aa) fusion proteins were both successfully applied in the immunoassay based on biotin-protein A and streptavidin-HR-P, and could detect the anti-PreS1(21-47aa) antibody with high sensitivity in sera from hepatitis B patients. The anti-PreS1(21-27aa) antibody was detected during the recovery phase of acute hepatitis B patients, but it was found only in few of the chronic carriers by the established conventional system. Conclusions: The follow-up study suggested that the presence of the anti-PreS1(21-27aa) antibody correlated well with the recovery of patients from hepatitis and the improvement in health. (C) 2002 Elsevier Science B.V. All rights reserved.
学科主题	Medical Laboratory Technology
类目[WOS]	Medical Laboratory Technology
关键词[WOS]	LARGE SURFACE-PROTEINS ; RECEPTOR-BINDING SITE ; PRE-S RESPONSES ; VIRUS-INFECTION ; MONOCLONAL-ANTIBODIES ; ENVELOPE ; SPECIFICITY ; ANTIGEN
收录类别	SCI
语种	英语
WOS记录号	WOS:000173895900019
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2554]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wei, J,Liu, XJ,Wang, YQ,et al. Development of the diagnostic immunoassay to detect anti-PreS1(21-47aa) antibody - a marker suggesting the health improvement of hepatitis B patients[J]. CLINICA CHIMICA ACTA,2002,317(1):159-169.
APA	Wei, J.,Liu, XJ.,Wang, YQ.,Lu, ZM.,Li, GD.,...&Zhang, ZC.(2002).Development of the diagnostic immunoassay to detect anti-PreS1(21-47aa) antibody - a marker suggesting the health improvement of hepatitis B patients.CLINICA CHIMICA ACTA,317(1),159-169.
MLA	Wei, J,et al."Development of the diagnostic immunoassay to detect anti-PreS1(21-47aa) antibody - a marker suggesting the health improvement of hepatitis B patients".CLINICA CHIMICA ACTA 317.1(2002):159-169.