The pH-dependent catalytic reaction of penicillin G acylase and its mutants

	The pH-dependent catalytic reaction of penicillin G acylase and its mutants
	Chen, JB; Yang, S; Wu, XJ; Li, SY; Yuan, ZY
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2002
卷号	34 期号:6 页码:786-789
关键词	catalytic reaction mechanism penicillin G acylase kinetic method ionizing residues
通讯作者	,
英文摘要	The pH-dependence in the catalytic reaction of recombinant penicillin G acylase and its mutants from B. megaterium has been studied by using kinetic methods. pK1 and pK2 of the residues of the wild type penicillin G acylase, involved in the catalyzed reaction, were 5.50-5.87 and 10.73, respectively, from the curves of logV(m) and log(V-m/K-m) versus pH. Results showed that the pK1 and pK(2)values of these residues of the mutants were similar to that of the wild type. pK(1) of 5.64-5.86 for mutant A and 5.69-6.96 for mutant B were obtained, while pK2 was 10.61 and 10.48 for mutant A and B, respectively. At the same time, pK values at different temperatures were investigated. The ionization enthalpies(OH) were 44.38-59.03 kJ/mol and 147.37 kJ/mol, respectively, from the curve of pK versus temperature. It was presumed according to the results mentioned above that the ionizing residues, involved in the reaction, were histidine and lysine that are localized around the active site.
学科主题	Biochemistry & Molecular Biology ; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	STABILITY
收录类别	SCI
语种	中文
WOS记录号	WOS:000179453000020
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2520]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Chen, JB,Yang, S,Wu, XJ,et al. The pH-dependent catalytic reaction of penicillin G acylase and its mutants[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(6):786-789.
APA	Chen, JB,Yang, S,Wu, XJ,Li, SY,&Yuan, ZY.(2002).The pH-dependent catalytic reaction of penicillin G acylase and its mutants.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(6),786-789.
MLA	Chen, JB,et al."The pH-dependent catalytic reaction of penicillin G acylase and its mutants".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.6(2002):786-789.