Over-expression of LPTS-L in hepatocellular carcinoma cell line SMMC-7721 induces crisis

	Over-expression of LPTS-L in hepatocellular carcinoma cell line SMMC-7721 induces crisis
	Liao, C; Zhao, MJ; Zhao, J; Jia, D; Song, H; Li, ZP
刊名	WORLD JOURNAL OF GASTROENTEROLOGY
	2002
卷号	8 期号:6 页码:1050-1052
通讯作者	Zhao, MJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,
英文摘要	AIM: To evaluate the function of the longer transcripts LPTS-L in hepatocellular carcinoma cell line SMMC-7721. METHODS: SMMC-7721 cells were transfected with LPTS-L expression construct and stably transfected cells were selected by G418. Multiple single clones formed and were checked for their phenotype. In the study of the effect on telomerase activity of LPTS-L in vitro, GST-LPTS-L fusion protein was expressed in E.coli and purified by glutathione-agarose column. Telomeric repeat amplification protocol (TRAP) assays were performed to study the influence of telomerase activity in SMMC-7721 cells. RESULTS: Over-expression of LPTS-L induced SMMC-7721 cells into crisis. LPTS-L could inhibit the telomerase activity in SMMC-7721 cells in vitro. CONCLUSION: LPTS-L is a potent telomerase inhibitor. Over-expression of LPTS-L can induce hepatoma cells into crisis due to the reduction of telomerase activity.
学科主题	Gastroenterology & Hepatology
类目[WOS]	Gastroenterology & Hepatology
关键词[WOS]	TELOMERASE ACTIVITY ; CANCER ; IDENTIFICATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000179741700018
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2516]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liao, C,Zhao, MJ,Zhao, J,et al. Over-expression of LPTS-L in hepatocellular carcinoma cell line SMMC-7721 induces crisis[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2002,8(6):1050-1052.
APA	Liao, C,Zhao, MJ,Zhao, J,Jia, D,Song, H,&Li, ZP.(2002).Over-expression of LPTS-L in hepatocellular carcinoma cell line SMMC-7721 induces crisis.WORLD JOURNAL OF GASTROENTEROLOGY,8(6),1050-1052.
MLA	Liao, C,et al."Over-expression of LPTS-L in hepatocellular carcinoma cell line SMMC-7721 induces crisis".WORLD JOURNAL OF GASTROENTEROLOGY 8.6(2002):1050-1052.