Identification and comparative analysis of differentially expressed proteins in rat striatum following 6-hydroxydopamine lesions of the nigrostriatal pathway: up-regulation of amyloid precursor-like protein 2 expression

	Identification and comparative analysis of differentially expressed proteins in rat striatum following 6-hydroxydopamine lesions of the nigrostriatal pathway: up-regulation of amyloid precursor-like protein 2 expression
	Shen, Y; Yu, Y; Guo, H; Tang, ZS; Yu, FSX; Zhou, JW
刊名	EUROPEAN JOURNAL OF NEUROSCIENCE
	2002
卷号	16 期号:5 页码:896-906
关键词	amyloid-precursor-like protein 2 dopaminergic nigrostriatal system gel electrophoresis Parkinson's disease striatum
通讯作者	Zhou, JW (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Proteom, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,
英文摘要	During neurodegenerative processes, cascades of degeneration and subsequent regeneration are triggered. However, the molecular nature of the factors involved in the neurodegeneration of the CNS remains largely unknown. In this study, the variations of protein expression in the striatum of adult Sprague-Dawley rats following 6-hydroxydopamine lesions were investigated, in order to better understand the molecular events occurring in the denervated target tissue. The rat striatum, ipsilateral to the lesion was analysed by two-dimensional gel electrophoresis followed by matrix assisted laser desorption/ionization-time of flight mass spectrometry. Seven proteins were up-regulated (188.1-750% compared to control) in response to the lesion: amyloid precursor-like protein 2 (APLP2), kininogen, glucokinase, tropomyosin alpha chain, type brain-1 and calpactin I light chain; whilst four proteins, neural epidermal growth factor-like 2, minichromosome maintenance 6, and thyroid hormone receptor beta-2, were down-regulated (to between 36% and 59% of levels in sham-operated controls). Three proteins that did not match with available data in the SWISS-PROT protein database were also determined. Immunohistochemical analysis demonstrated colocalization of APLP2 and tyrosine hydroxylase in the nigral neurons. Moreover, reduction of APLP2-positive neurons in the substantia nigra pars compacta as well as the increases in the substantia nigra pars reticulata and in the striatum were observed. Furthermore, the conditioned medium of the Chinese hamster ovary cells over-expressing APLP2-751 (chondroitin sulphate-modified), but not APLP2-763 (nonchondroitin sulphate-modified), was able to increase the number of the tyrosine hydroxylase-positive neurons in fetal mesencephalic cultures. These results suggest that the expression of APLP2, a protein that has been thought to be associated with Alzheimer's disease, is up-regulated in the striatum following dopaminergic denervation. They also support the view that chondroitin sulphate-modified APLP2 protein may play an important role in the dopaminergic nigrostriatal system.
学科主题	Neurosciences & Neurology
类目[WOS]	Neurosciences
关键词[WOS]	CHONDROITIN SULFATE GLYCOSAMINOGLYCAN ; ALZHEIMERS-DISEASE ; PARKINSONS-DISEASE ; MESSENGER-RNAS ; VENTRAL MESENCEPHALON ; CEREBROSPINAL-FLUID ; NEUROTROPHIC FACTOR ; NEURITE OUTGROWTH ; INHIBITOR DOMAIN ; BASAL GANGLIA
收录类别	SCI
语种	英语
WOS记录号	WOS:000178538600014
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2509]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Shen, Y,Yu, Y,Guo, H,et al. Identification and comparative analysis of differentially expressed proteins in rat striatum following 6-hydroxydopamine lesions of the nigrostriatal pathway: up-regulation of amyloid precursor-like protein 2 expression[J]. EUROPEAN JOURNAL OF NEUROSCIENCE,2002,16(5):896-906.
APA	Shen, Y,Yu, Y,Guo, H,Tang, ZS,Yu, FSX,&Zhou, JW.(2002).Identification and comparative analysis of differentially expressed proteins in rat striatum following 6-hydroxydopamine lesions of the nigrostriatal pathway: up-regulation of amyloid precursor-like protein 2 expression.EUROPEAN JOURNAL OF NEUROSCIENCE,16(5),896-906.
MLA	Shen, Y,et al."Identification and comparative analysis of differentially expressed proteins in rat striatum following 6-hydroxydopamine lesions of the nigrostriatal pathway: up-regulation of amyloid precursor-like protein 2 expression".EUROPEAN JOURNAL OF NEUROSCIENCE 16.5(2002):896-906.