Biphasic effect of GABA on rat sperm acrosome reaction: Involvement of GABA(A) and GABA(B) receptors

	Biphasic effect of GABA on rat sperm acrosome reaction: Involvement of GABA(A) and GABA(B) receptors
	Hu, JH; He, XB; Wu, Q; Yan, YC
刊名	ARCHIVES OF ANDROLOGY
	2002
卷号	48 期号:5 页码:369-378
关键词	baclofen capacitation chlortetracycline staining fertilization saclofen
通讯作者	Yan, YC (reprint author), Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Mol Cell Biol Lab, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	The functional relationship between GABA(A) and GABA(B) receptors in regulating aerosome reaction (AR) of rat spermatozoa was demonstrated by studying the differential effects of a GABA(B) agonist and an antagonist on the process. AR rates were determined using the chlortetracycline staining assay. The induction of AR in rat sperm by GABA Was found to be a biphasic phenomenon i.e., AR rates increased with increasing GABA concentrations tip to <5 muM and at higher concentrations of the neurotransmitter (>5 muM), there was a reduction in the AR rates. This biphasic phenomenon is apparently due to the differential interaction of the neurotransmitter with GABA receptor subtypes in a dose-dependent manner; i.e., GABA(A) receptors (stimulatory) are primarily activated at low concentration of GABA, while GABA(B) receptors (inhibitory) become activated at higher concentrations. This hypothesis is supported by the present findings that treatment with saclofen, a GABA(B) receptor antagonist, did not influence the AR rates effected by GABA at low concentrations; while the A R rates were maintained at the maximum level at higher concentrations of GABA, resulting in the elimination of the biphasic phenomenon. Baclofen, a GABA(B) receptor agonist, blocks the AR activating action of GABA at both low and high concentrations. It would appear that the induction of AR in rat sperm by GABA is regulated by the proportionality of activated GABA(A) and GABA(B) receptors acting as a yin-yang control.
学科主题	Endocrinology & Metabolism
类目[WOS]	Andrology
关键词[WOS]	GAMMA-AMINOBUTYRIC-ACID ; MOUSE SPERMATOZOA ; CALCIUM CHANNELS ; MAMMALIAN SPERM ; PLASMA-MEMBRANE ; ZONA-PELLUCIDA ; PROGESTERONE ; CAPACITATION ; TRANSMITTER ; ACTIVATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000177524100007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2508]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Hu, JH,He, XB,Wu, Q,et al. Biphasic effect of GABA on rat sperm acrosome reaction: Involvement of GABA(A) and GABA(B) receptors[J]. ARCHIVES OF ANDROLOGY,2002,48(5):369-378.
APA	Hu, JH,He, XB,Wu, Q,&Yan, YC.(2002).Biphasic effect of GABA on rat sperm acrosome reaction: Involvement of GABA(A) and GABA(B) receptors.ARCHIVES OF ANDROLOGY,48(5),369-378.
MLA	Hu, JH,et al."Biphasic effect of GABA on rat sperm acrosome reaction: Involvement of GABA(A) and GABA(B) receptors".ARCHIVES OF ANDROLOGY 48.5(2002):369-378.